Name ____________________________________________________________ The Onion’s Tip (from the Biology Project, University of Arizona) In this activity, you will be presented with cells from the tip of an onion root. Using your notes, you will classify each cell based on what phase it is in. At the end you will count up the cells found in each phase and use those numbers to predict how much time a dividing cell spends in each phase. You can base your calculation on a total cell cycle of 24 hours. Enter data in this table below. Interphase Prophase Metaphase Anaphase Telophase Total Number of Cells 36 Percent of Cells 100% Examples of pictures of cells in each phase: Interphase - In interphase, the chromosomes are not condensed yet, and individual chromosomes cannot be distinguished. Prophase – Chromosomes in prophase are visible, but are not organized. Metaphase – Cells in metaphase have distinct chromosomes that are lined up along the middle of the cell. Anaphase – In anaphase, the sister chromatids separate and migrate to opposite poles of the cell. Telophase – In telophase, the sister chromatids have been pulled to opposite sides of the cell and the nuclear membrane forms around each new nucleus. Interphase Prophase Metaphase Anaphase Telophase Identify which phase the cell is in the pictures below: Phase Phase Phase Phase Phase Phase Phase Phase Prophase (spindle) Telophase Telophase (chromosome/ centromere) Interphase Metaphase (spindle fiber) Anaphase Telophase Interphase (nuclear membrane, nucleolus, and centriole) Phase Color Anaphase – the phase of mitosis in which the chromosomes begin to separate. Centrioles – paired cylindrical organelles, arranged at right angles to each other, located at the center of a microtubule. Centromeres - a centromere is the constricted region of a nuclear chromosome - microfibers attach to the centromere during mitosis. Chromosomes - structures in the nucleus that contain DNA molecules that contain the genes. Interphase - the phase of a cell's life cycle in which DNA is replicated. Microtubules - tiny filaments (about 25 nanometers in diameter) that are active in mitosis. Metaphase - the phase of mitosis in which the chromosomes line up at the equator (the central plane) of the cell. Prophase - the phase of mitosis in which the duplicated chromosomes condense, the nuclear envelope dissolves, and centrioles divide and move to opposite ends of the cell. Telophase - the last phase of mitosis, when the chromosomes migrate to opposite ends of the cell, two new nuclear envelopes form, and the chromosomes uncoil. Name _______________________________________________ Score ________ The Magic Universe of Cells: How Many Cells are Born in a Day? Materials Calculator Safety Concerns: None Procedure Your cell divides every hour. Assuming no cells die during the 12-hour period, how many cells will exist after each hour? How many cells would exist after 12 hours? After you use the chart below as a worksheet, graph your results on the back of this sheet of paper. One axis of the graph should represent the number of cells and the other axis should represent the hours up to the 12th hour. Cell Division Chart Hour 1 2 Number of Cells 3 4 5 6 7 8 9 10 11 12 Look at your graph. What pattern do you notice?