Lesson Plan Summary Sheet
Teacher:
Howerton
Subject:
Biotech 2
Topic:
Unit A: Laboratory Research Techniques
Day
8/25
Essential Question
What are the course expectations?
8/26
What basic information is needed to
do research in a biotech lab?
What are the proper procedures for
maintaining a laboratory notebook?
8/27
8/28-29
What lab safety procedures should be
followed in a biotech lab?
What are the proper procedures for
using pipettes and balances in the
lab?
What are the proper procedures for
using spectrophotometers in the lab?
What are the proper procedures for
using pH meters in the lab?
Activity: Students will:
□ Introduce the course and explain the
expectations
□ Take a Pre-Test
□ View notes on Lab Notebook and Safety
Powerpoint
□ View a Lab Safety Video
□ Complete Lab 1: Lab Safety and the
Notebook
Vocabulary
none
Learning Log
none
varies
GLP
SOP
MSDS
PPE
Aseptic techniques
A1: Explain the
importance of having
procedures regarding
safety and your
notebook.
□
□
Calibration, accuracy,
precision, significant digits
A2: Write an SOP for use a
micropipette or balance.
pH, buffer, dilution,
Absorbance, transmittance,
refelction, wavelength,
optical density, Beer’s
Law, blank
Gram staining
A3: Write an SOP for using
the spectrophotometer or pH
meter.
□
□
Review Metrology powerpoint
Complete Lab 2:Verifying your
Micropipets
Review Lab Skills Recap powerpoint
Complete Lab 3: Protein Concentration
Determination
9/2
How do you use gram staining to
characterize bacteria?
□
□
View Microbiology powerpoint
Complete Lab 4: Gram Staining
9/3
How can the antibiotic resistance of
bacteria be studied in the lab?
□
□
View Antibiotics powerpoint
Complete the Lab 5: Antibiotics Assay
Antibiotics, selection,
resistance
9/4-5
How are cells cultured in a biotech
lab?
□
□
View Cell Culturing powerpoint
Complete Lab 6: Cell Culturing.
9/8
9/9
How are fermentation reactors set
up?
What steps must be followed to
design and report on a scientific
experiment?
□
□
□
□
View a Fermentation powerpoint
Set up the Lab 7: Fermentation
View the Scientific Method powerpoint
Design and carryout Lab 8:
Experimenting with Physarum and Lab
9: Finding Antibiotic Activity
9/10
What basic techniques are used to do
□
Wrap-up and review
Hemocytometer
Inverted microscope
Viability
Fermentation
Bioprocessing
Question
Hypothesis
Design and testing
Conclusion
Literature cited
No new vocabulary
A4: Describe a gram positive,
streptococcus bacteria would
appear under the microscope.
A5; Explain how bacteria can
gain antibiotic resistance in
the lab.
A6: Explain what is necessary
to culture both bacterial and
mammalian cells.
A7: Explain the process of
fermentation
A8: Give an example of how
the scientific method can be
used in everyday life outside
the science classroom.
A9: Explain how you plan to
9/11
research in a biotech laboratory?
□
Update lab notebook by completing all lab
write ups
What basic techniques are used to do
research in a biotech laboratory?
□
Test on Unit A: Laboratory and
Research Techniques
Turn in Summarizing Strategies ( or no
test corrections
Turn in Lab Notebook
Students will see some examples of what
to do and not do when doing their literature
research
Visit FTCC Biotech Program
□
How do a good literature research on
your science project topic?
9/12
□
□
□
No new vocabulary
study for this test and then
whether you achieved your
goals.
A10: Write a reflection on
your effort during this unit and
what you have learned.