Ap Biology Biotech Test study guide Biotech test includes all of the techniques and concepts + bacteria and viruses (videos on Ustream and on links page) Techniques to know: (know purpose, how, and how to interpret) gene cloning (including putting genes in vectors, use of vectors host cells, and selection factors) genetic engineering (including how restriction enzymes work) cDNA library formation cDNA PCR Electrophoresis, Southern Blotting, Probing vs. staining gel, and uses of electrophoresis like paternity testing and DNA fingerprinting Sequencing MicroArray analysis Transgenics Cloning of Organisms Stem Cell Research For viruses - just know basic structure, how they infect, and general types especially retroviruses and how they work For bacteria - know the 4 ways that give bacteria a lot of variation even though they reproduce asexually (know what each is and how it works) mutation, transformation, transduction, conjugation Know basic bacteria structure (no need to know gram +- stuff), plasmids