CHINESE MEDICINAL BIOTECHNOLOGY
Bimonthly
Established in December 2006
Responsible Institution
National Health and Family
Planning Commission of the
People's Republic of China
Sponsor
China Medicinal Biotech Association
1011 Guangqujiayuan 2th Building,
Dongcheng District, Beijing 100022, China
Editing
Editorial Board of Chinese
Medicinal Biotechnology
Institute of Medicinal Biotechnology,
Chinese Academy of Medical Sciences
1#, Tian Tan Xi Li, Beijing, 100050, China
Email: cmbj01@126.com
http://www.cmbp.net.cn
Editor-in-Chief
JIANG Jian-dong（蒋建东）
Managing Director
SHENG Feng-nian（盛丰年）
Publishing
Publishing House,
Chinese Medicinal Biotechnology
2# Nanwei Road, Xicheng District, Beijing
100050, China
Tel: 0086-10-62115986
Fax: 0086-10-62115976
Email: cmbj01@126.com
Printing
Beijing Zhongke Printing Limited
Company
Overseas Distributor
China International Book
Trading Corporation
P. O. Box 399, Beijing, China
Code No. BM4275
Mail-Order
Publishing House,
Chinese Medicinal Biotechnology
2# Nanwei Road, Xicheng District, Beijing
100050, China
Tel: 0086-10-62115986
Fax: 0086-10-62115976
Email: cmbj01@126.com
CSSN
ISSN 1673-713X
CN 11- 5512 / R
Copyright© 2013 by the Publishing
House, Chinese Medicinal
Biotechnology
All articles published represent the
opinions of the authors, not reflecting
the official policy of the China
Medicinal Biotech Association or the
Editorial Board of Chinese Medicinal
Biotechnology, unless this is clearly
specified.
Volume 9, Number 1
February 10, 2014
CONTENTS OF SELECTED ARTICLES
Editorial
Mystery of vesicle transport
—— review of 2013 Nobel Prize in Physiology or Medicine…………… 2
FANG Zheng-yu, LI Gang
Original Articles
Construction of retargeting helper adenovirus and its functions …………… 6
YANG Yue-feng, LI Ze-liang, LU Zhuo-zhuang, WANG Hua, XIAO Feng-jun,
ZHANG Qun-wei, SUN Hui-yan, WANG Li-sheng
An improved method to generate site-specific integration
minicircle DNA ………………………………………………………………… 13
LIU Liu, ZHOU Zai-wei, MA Qing-wen
Effect of HE4 gene silenced by siRNA on the proliferation
and invasiveness of ovarian cancer cell SK-OV-3 ………………………… 20
FANG Qing, WANG Zai, YOU Jia, ZHAN Xue-mei, YANG Ai-lian, WEI Ji-hong, FANG Zhao
Screening of potential inhibitors of L12-L10 interaction
with antituberculosis activity ………………………………………………… 26
LI Yan, ZHANG Xue-lian, LI Yong-zhen, LI Dong-sheng, YOU Xue-fu, SI Shu-yi
The clinical significance of expression of macrophage migration
inhibitory factor in urothelial carcinoma of the bladder ……………… 32
FENG Liu-wei, LI Tie-qiang, HOU Jun-qing
The overexpression of tribbles homolog 3 promotes the secretion
of collagen I in mouse fibroblast cell ……………………………………… 37
LIU Hong, XIE Qiong, LI Yong-zhi, XUE Chun-mei, WANG Jing, WANG Jia-ping
Efficient preparation of human HPV16 L1 virus-like particles
in prokaryotic system …………………………………………………………… 43
XIE Xiao-lu, WEI Dong, WANG Guo-zhi, HUANG Chang-jiang
Reviews
Overview of 3-hydroxy-3-methylglutaryl coenzyme A lyase (HMGL)
involved in mevalonate metabolism pathway ……………………………… 48
WANG Zhi-biao, WANG Wei, CHENG Ke-di, KONG Jian-qiang
Research progress of mammalian cell culture medium
for antibody drug production ………………………………………………… 53
DUAN Xu-jie, REN-tong, LUO Hou-yong, LIU Rui, XU Wei-tao
Research progress of sample pre-treatment methods
for urine proteomics …………………………………………………………… 58
LU Hai-tao, NIU Chao, NI Mao-wei, ZHU Zhong-xin, CONG Wei-tao, JIN Li-tai
Research advances in effects and mechanisms of inflammatory state
on CYP2B6 activity …………………………………………………………… 62
SUN Hai-yan
Technology and Method
Measurement for residual DNA in human recombinant neutrophil
inhibitory factor and hirulog hybrid (TNHH) host
using digoxin labeled probe …………………………………………………… 66
CAI Xiao-na, WANG Peng, HE Xiao-qiang, LIU Li-ping, CHEN Hai-rong,
GUO Jin-xia, FAN Kai
Verification of storage period for Bacillus subtilis spore suspension …… 69
XIAO Huang, LIN Ji-heng, MA Shi-hong, HU Chang-qin