CHINESE MEDICINAL BIOTECHNOLOGY Bimonthly Established in December 2006 Volume 6, Number 6 December 10, 2011 Responsible Institution Ministry of Health of the People’s Republic of China CONTENTS OF SELECTED ARTICLES Sponsor China Medicinal Biotech Association Editorial New development of in vitro detection and diagnosis technology for disease ……………………………………………………………………… 401 1011 Guangqujiayuan 2th Building, Dongcheng District, Beijing 100022, China Editing Editorial Board of Chinese Medicinal Biotechnology Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences 1#, Tian Tan Xi Li, Beijing, 100050, China Email: cmbj01@126.com http://www.cmbp.net.cn Editor-in-Chief ZHAO Kai(赵铠) Managing Director SHENG Feng-nian(盛丰年) Publishing Publishing House, Chinese Medicinal Biotechnology 1011 Guangqujiayuan 2th Building, Dongcheng District, Beijing 100022, China Tel: 0086-10-62115986 Fax: 0086-10-62115976 Email: cmbj01@126.com Printing Beijing Zhongke Printing Limited Company Overseas Distributor China International Book Trading Corporation P. O. Box 399, Beijing, China Code No. BM4275 Mail-Order Publishing House, Chinese Medicinal Biotechnology 1011 Guangqujiayuan 2th Building, Dongcheng District, Beijing 100022, China Tel: 0086-10-621159869 Fax: 0086-10-62115976 Email: cmbj01@126.com CSSN ISSN 1673-713X CN 11- 5512 / R Copyright© 2011 by the Publishing House, Chinese Medicinal Biotechnology All articles published represent the opinions of the authors, not reflecting the official policy of the China Medicinal Biotech Association or the Editorial Board of Chinese Medicinal Biotechnology, unless this is clearly specified. TANG Shi-xing, KANG Ke-ren, LI Yin-tai Biological Diagnostic Technology The detection and comparison of neutralizing antibodies response to Varicella-Zoster virus in domestic plasmapheresis donations using two commercial ELISA kits ………………………………………… 405 WANG Zhuo, TANG Qian, LV Mao-min, MA Yu-yuan, ZHAO Xiong, ZHANG Jin-gang Correlation between WISP-1/NGX6 gene expression and cervical cancer cell proliferation and/or metastasis analyzed by real-time fluorescence quantitative PCR ……………………………… 410 QIN Jie, CHEN Jin, HUANG Shou-guo Expression and purification of recombinant HIV-1 gp41 protein and determination of its immunoreactivity ……………………………… 415 LIANG Quan, LUO Chun-zhen, MAO Pei-ju, WANG Man A review of diagnostic technology on major infectious disease ………… 420 XU Si-hong, WANG You-chun The status and progress of gram staining …………………………………… 424 YE Yuan-kang Loop-mediated isothermal amplification technology for the detection of influenza virus ……………………………………………………………… 426 LIU Juan, JIANG Tao, QIN E-de, QIN Cheng-feng Influenza A virus subtype H1N1 (2009) and the development and clinical application of its nucleic acid diagnostic reagent ………… 429 LI Lin, GE Jun, CAO Jian-rong Original Articles SopB, an effector of Salmonella, activates the phosphorylation of MAPK in HeLa cells ……………………………………………………… 432 TAO Yong-qing, LIU Chong-ji, LI Jian-jiao, XU Xin, WANG Jin-han, HAN Na, ZHANG Lei, ZHAO Hui, RUAN Hai-hua, WANG Chang-lu A zebrafish model for liver-specific expression of HCV NS5A gene …… 437 ZHAO Ye, HU Zhan-ying, TONG Jun-wei, ZHAO Li-xun, JIANG Jian-dong, ZHANG Jing-pu Establishment and application of a novel high-throughput screening model targeting to inosine monophosphate dehydrogenase for antitubercular drugs ……………………………………………………… 445 XIONG Xiao-jiao, ZHOU Shuang, YANG Yan-hui, GUAN Yan, XIAO Chun-ling Protective effect of baicalin spray on complete cerebral ischemia in rats …………………………………………………………………………… 450 SHI Ya-jun, SHI Jun-hui, LIU Jian-yun, TANG Mei, HUANG Qin-wan, YANG Ming Reviews Recognition properties of nucleic acid to toll-like receptors and its role in systemic lupus erythematosus ……………………………… 455 WEI Wei, ZHANG Yi-liang, GUO Ying-jun, HUANG Jin-feng, SUN Shu-han DNA methylation and heart failure …………………………………………… 459 ZHOU Yun-guo, HONG Kui