Reg. No. ________
Karunya University
(Karunya Institute of Technology and Sciences)
(Declared as Deemed to be University under Sec.3 of the UGC Act, 1956)
End Semester Examination – November/ December - 2012
Subject Title:
Subject Code:
DOWNSTREAM PROCESSING
09BT222
Time: 3 hours
Maximum Marks: 100
Answer ALL questions
PART – A (10 x 1 = 10 MARKS)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
What are high value, low volume, and high purity products?
What are the characteristics features of bioprocesses?
What is a gyro tester?
Define filtration.
Expand CSTR.
Define reverse osmosis.
What is retention time?
What is pseudoaffinity chromatography?
What are the steps involved in finishing operation of bioproducts?
What is lyophilization?
PART – B (5 x 3 = 15 MARKS)
11.
12.
13.
14.
15.
Outline the different steps in the downstream processing of biochemical products.
Discuss the separation of solids by tubular bowl centrifuge.
Write a note on classification of membrane separation processes.
Write the applications of reversed phase chromatography.
Explain about final product formulation.
PART – C (5 x 15 = 75 MARKS)
16. Write a detailed account on chemical, enzymatic and mechanical methods of cell disruption.
(OR)
17. a. What are protein inclusion bodies? How are they converted to native proteins?
(7)
b. Explain the methods of pretreatment of fermentation broth.
(8)
18. a.
b.
Describe the different equipments used for filtration process with a neat diagram.
(10)
Write a note on Centrifugal filtration.
(5)
(OR)
19. The centrifugal separation of a biomass of 80µm-sized cells of density 1.04kg m-3 was carried
out in a tubular centrifuge having a diameter of 15cm and rotating at 1200 rev/min.
a. Calculate the residence time if the distance between the liquid surface and the axis of
rotation was 0.8 cm, the liquid density and the liquid viscosity were 1.0 kg m-3 and 0.013g
cm-1 s-1 respectively.
b. What would be the volumetric capacity of the centrifuge if its length was 40 cm?
c. Calculate the ∑ factor.
d. What will be the time required for centrifuging 1000 liters of broth?
20. Illustrate the principle, extraction process, equipment and applications of aqueous Two – Phase
extraction.
(OR)
[P.T.O]
21. Elucidate on the Protein precipitation methods.
22. What is ion - exchangers? Explicate the principle, operating modes and practice of ion exchange chromatography.
(OR)
23. Explain the following:
a. Gel filtration
b. Bioaffinity chromatography
(8+7)
24. Discuss briefly about different types of drying equipments and freeze-drying.
(OR)
25. Write a detailed account on crystallization theory, crystallization practice and equipments used
for crystallization.