Zagazig University
Faculty of Pharmacy
Graduate program
Analytical chemistry department
Part A
Instrumental analysis (II)
Time 3 h.
19/7/2012
Two parts
Q1 Write short note on three only of the following:
1234-
Location of separated substance on plane chromatography.
Types of paper used in paper chromatography.
Principles of electrophoresis, explaining the factors affecting migration rate.
Explained the principle of GLC chromatography
Q2 Define the following:
1.
2.
3.
4.
(3x 10 30 marks)
(4 x 2.5 = 10 marks)
Minimum detection limits in GLC
Linearity
Response
Enumerate the most common detectors used in GLC
Part B
Q 3: Write on the following:
(15 x 4 = 60 marks)
1. SCE electrode, (mention: half reaction, half cell, design, Nernst equation,
advantages and disadvantages of the electrode if available)
2. Factors affecting the solution conductance
3. Conductometric titration curve of mix of NaCl # AgNO3
4.Types of transducer in spectrophotometer
5.The method of continuous variations for determination of metal ligand
complex
6.Effect of Concentration on Fluorescence Intensity.
7. Electrothermal or graphite furnace atomizer
8. Atomizer and atomization process
9. Hollow-cathode Lamps
10. Differences between fluorescence and phosphorescence.
11.
12.
13.
14.
15.
Methods of detection end point potentiometrically
Photomultiplier or photo emissive tube
Effect of Concentration on Fluorescence Intensity.
Advantages and disadvantages of atomic absorption spectroscopy
IR spectrophotometers.