Teaching Unit 7 Separation techniques: column chromatography Keywords and concepts: Mixture separation. Column chromatography. ’Xplore Health related tools: Virtual experiment: Develop a drug! Introduction: In our day-to-day we use devices that are based on some techniques that are also used in the lab. In this teaching unit you will learn how a separation technique such as column chromatography is used for water filtration. ACTIVITY 1 In the virtual experiment “Develop a drug” a high performance liquid chromatography (HPLC) is used to separate the POP protein from the rest of bacterial proteins. HPLC is a column chromatography based on the affinity of molecules for the support or the solvent and it works in a similar way as that of water filtration systems that can be found in some homes to get rid of substances that give a bad taste to tap water. In these systems, filtration is based on chemical bonding. Please identify which of the following drawings corresponds to the HPLC used in the virtual experiment and which corresponds to the process used in filtration systems. Explain in your own words the differences between both processes. AUTHOR: FUNDED BY: Teaching Unit 7 -1-