Floral Park Memorial High School
The Sewanhaka Central High School District
NAME:_____________________________________
DATE:________________________________
Mrs. Naus
Forensic Science
Chapter 5 Take Home Exam
Directions:Place all of your answers IN PEN on the exam answer sheet at the end
1- Any type of matter that does NOT contain the element carbon is considered:
2-Where in the periodic table are the heaviest elements located?
3- A”Fingerprint “ of a paint sample can be made by the process of ________.
4- When separating black ink in the process of paper chromatography, the
different colored bands are created by the different _________________ of the
molecules.
5- A “fingerprint” of a substance such as heroin can be created through the
process of: write out the letter and answer on your answer sheet
a- gas chromatography
b- gel electrophoresis
c- infrared spectrogram
d- light dispersion
6- Definition: the simplest substance known provides the building blocks for all
matter.
Use the picture below to answer questions 7-9
Picture A
Picture B
Picture C
7- Picture C: write out the letter and answer on your answer key
A- has definite shape and definite volume
B- has no definite shape but takes on the volume of its container
C- has no definite shape or volume
D- has definite volume but takes on the shape of its container
8- The atomic arrangement of a gas is most likely represented by which picture?
9- Identify the phase of matter and define its shape and volume for Picture B.
10- A charged atom is defined as:_____________
Use the scenerio below to answer questions 11 and 12
A dead fisherman is found floating belly up down the east river. A vile of white
liquid is found in his pocket. After performing an infrared spectrogram the liquid is
determined to be 92% rat poison and 8% ether.
11- What is the qualitative conclusion of this substance?
12- What is the quantitative conclusion of this substance?
13- In the chemical reaction between sodium (Na) and chloride (Cl), sodium
gives up an electron to chloride therefore forming ___________________. Write
both the letter and the answer on your answer sheet
A- atom
B- element
C- ion
D- solid
14- Which phase change changes a solid to a gas?
15- _____________________ is defined as the ability to do work.
16- All methods of chromatography contain both a _____________ and _______________
phase.
17- IN YOUR OWN WORDS, DEFINE CHROMATOGRAPHY.
Use the picture below to answer questions 18 and 19
Solvent front
Ether
Rat Poison
Origin
1
2
3
18- As the solvent moves
through the paper, which
solute dissolves easier and explain how you know this.
19- Can you identify the contents of the unknown sample displayed in column 3
and if so explain how.
20- List the four properties used to separate a solute in the process of
chromatography.
21- Before injecting a sample into a gas chromatography column it must be
heated or __________________ in order to make it a __________________.
22-Solutes that display polarity and interact with the stationary portion in an
HPLC column have: Write your letter choice and answer on your answer sheet
A- A smaller Rf value due to the distance they have to travel
B- A smaller retention time due to the size of the molecules
C- A smaller retention time but a greater Rf value
D- A greater retention time due to the speed of the molecules
Use the gas chromatogram below to answer questions 23-25
23- Qualitative analysis of Methane is determined by its: Write out the letter and
answer on your answer key
A- Peak height
B- retention time
C- peak width
D- Choices A and C NOT B
24- Which solute is most soluble in this chromatogram and how do you know?
What I mean is which molecules reacted best with the gas and how do you
know?
25- Explain how you quantitatively determine the concentration of each
substance.
26- Explain how Thin Layer chromatography is similar to paper
chromatography.
27- Define Rf value and explain when it is used.
28- As you move from gamma to radio rays, the wavelength (increases or
decreases) while the frequency of wavelengths (increase or decrease)
29- Which type of chromatography takes advantage of the ability to change the
temperature?
30- the function of restriction enzymes are to: Write out the letter and the answer
on your answer sheet.
A- to separate DNA fragments based on size and solubility
B- to locate and cut specific sequences of DNA
C- to cut a polypeptide into single amino acids
D- none of the above
31- Which process is commonly used to identify blood, proteins, DNA and
enzymes?
32- After white light is dispersed through a prism, which color has the largest
wavelength?
33- The only property different in each part of the electromagnetic spectrum is:
A- wavelength
B- light absorption
C- frequency D- spectrogram
Use the diagram below to answer questions 34and 35
34- Label each part of this blood sample.
1
35- Which part is most important to gel electrophoresis and why?
2
3
36- The fragments that migrate quickest towards the positive end of an
electrophoresis are: Write out the letter and answer on your answer sheet
A- small and positive
B- large and positive
C- large and negative
D- small and negative
Use the gel electrophoresis below to answer questions 37-40
Examine the diagram of an agarose gel below and answer the following questions.
37-What do the bands in the drawing represent?
38-Which bands traveled the slowest? (use the number)
39- If band A is found on the victim and bands B-E are the suspects can any of these
people be found guilty? Why or why not?
40-What causes the fragments to move through the gel?