C483 Study Guide for Exam 1 Fall 2015 Basic Information Exam 1: Thursday, September 24, 7:15-9:15PM in Woodburn Hall 101 Arrive early for assigned seats Bring your student ID. Failure to do so will result in getting your exam back later. You may use a NON-PROGRAMMABLE calculator. All papers, books, phones, and electronic devices must be in a sealed bag under your seat. Exam Content: The exam will cover chapters 1-6. All material covered in classnotes, book, and homework could be on the exam. Details from case studies will not be included, but problems of that sort are on the exam. Some major topics include: Solubility, free energy/enthalpy/entropy, coupled reactions, intermolecular forces, solvation, hydrophobic effect, amphipathic compounds, auto-ionization, pH, pKa, pH of strong acid solutions, weak acids, Henderson-Hasselbalch, ionization state, titration curves, buffer capacity, polyprotic acids, blood buffer, nucleobase structures, nucleoside, nucleotide, polynucleotide nomenclature, base pairing, basis of double helix stability, denaturation and melting, RNA structure/stability/function, transcription, translation, coding strand, Sanger method, PCR, recombinant DNA technology, nucleases, site directed mutagenesis, amino acid structures: stereochemistry and ionization state, essential amino acids, peptide bonds, disulfide bonds, structural effects of amide resonance, -helix, helical wheel, -sheets, loops and turns, motifs, domains, thermodynamics of protein folding, size exclusion chromatography, ion-exchange chromatography, affinity chromatography, SDS-PAGE, isoelectric focusing, Edman Degradation, partial digestion, myoglobin/hemoglobin structure-function, oxygen binding curve, hyperbolic vs sigmoidal curves, cooperativity, T vs R conformation, 2,3-BPG, Bohr effect, examples of fibrous structural proteins, microfilaments and microtubules, motor protein mechanisms, enzyme rate enhancement, recognizing 6 classes of enzyme catalyzed reactions, chemical mechanisms of catalysis, covalent catalysis, pH effects on enzymes, binding energy mechanisms, Lock and key vs induced fit, TS stabilization, chymotrypsin, catalytic triad, substrate specificity Exam Format: The exam is out of 100 points. The first 50 points will be fill in the blank, true/false, and short answer problems based on the Reading Guides. There will be four 10-point problems involving calculations, structural drawings, or short essay. There will be a 10 point case study. A bonus problem may or may not be included on the exam.