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MLS 2213
Course Title:
Clinical Chemistry II
Demonstrate written communication skills
Collect and safely handle biological specimens for analysis
Perform accurate laboratory testing of body fluids, cells, and other substances
Evaluate and interpret laboratory test data while recognizing factors that affect procedure and
1. Comply with established lab safety regulations
2. Perform total protein and albumin assays on at least two unknowns and controls with 95%
accuracy. Additionally, calculate the A/G ratios for these unknowns with 100% accuracy.
3. Interpret typical serum protein electrophoresis scans of at least two unknowns, with 100%
proficiency. Interpret acid and alkaline gel electrophoresis plates with 100% proficiency.
4. Explain the principles of the following techniques: Thin layer chromatography, column
chromatography, gel filtration chromatography, ion exchange chromatography, high
performance liquid chromatography.
5. Explain what special sample preparation steps must be taken to perform Urine Protein
electrophoresis and CSF protein electrophoresis.
6. Explain the principles of the following methods: total cholesterol (Liebermann-Buchard
method), Total Cholesterol (Cholesterol oxidase method), HDL cholesterol fractionation, LDL
cholesterol fractionation (direct and calculated), serum triglycerides, lipoprotein electrophoresis.
7. Define the following terms: enzyme, coenzyme, activator, isoenzyme.
8. List six variables which could lead to errors in enzyme assays.
9. Explain the principles of the following assay techniques used in Endocrinology studies:
radioimmunoassay, immunoradiometric assay, EMIT, ELISA, fluorescence polarization.
10. Describe the feedback regulatory mechanism for controlling the blood level of T3 and T4
hormones, involving the hypothalamus, anterior pituitary, and thyroid gland.
11. Differentiate between the clinical goals and purposes of TDM as opposed to toxicology.
I. Amino Acids and Proteins
II. Lipid Metabolism Studies
III. Clinical Enzymology
IV. Analytical Principles for Endocrinology
V. Endocrine Function Studies
VI. Therapeutic Drug Monitoring and Toxicology Studies
Professional Eval (5%)
Lab Report Forms (5%)
Lecture Exam 1 (15%)
Lecture Exam 2 (15%)
Lecture Exam 3 (15%)
Lecture Exam 4 (15%)
Final Exam (20%)
Lab Exam 1 (5%)
Lab Exam 2 (5%)
Clinical Laboratory Chemistry, Graves and Sunheim, Pearson, 2010
Areas in bold are required.