Chromatography review questions for exam III List a few other ways of separating compounds besides chromatography: What are the two basic components of any chromatography system? Vocabulary o Retention time o Resolution o Distribution coefficient o Theoretical plate What is the difference between analytical and preparative chromatography? Describe the terms in this equation: H = A + B/u + Cu List some ways you could increase the number of theoretical plates (N) List some ways you could reduce the retention time (make it go faster) Explain how gradient oven temperature profiles or gradient elution improve resolution and decrease retention time What is the typical mp in GC? How long can GC columns be compared to HPLC columns? Explain how the following GC detectors work o FID o ECD o TCD Why is it important to have uniform pores in the HPLC packing? Name the two most common HPLC detectors: How does an HPLC injector work Describe the chromatographic mechanism that allows analytes to separate in the following chromatography methods and which type of compound comes out first o GC/HPLC o Ion chromatography o Size exclusion o CE Draw a basic CE system How does CE work? Which van deemter (H = A + B/u + Cu ) terms are significant for CE and which are not ? Describe solute stacking What voltage range is used in CE? 10 kV to 50 kV ep V L2 L2 N Using the equation to the right, describe how to increase N 2 2 Dt 2D r Describe the two types of injection methods in CE and their advantages and disadvantages What sort of problems result when the mp heats up? What is good and bad about protein analysis in CE? What is the big deal about CE on a glass chip?