2015
ATTACHMENT F
Breeding Colonies
Complete one ATTACHMENT F for EACH STRAIN you are breeding.
Note that you are required to track and report the number of animals produced by in-house breeding.
Answer sections will expand.
Investigator Name:
Protocol Number:
Strain:
Strain name:
Genotype:
1.
Are the animals required for use in this protocol available from a commercial supplier?
YES
NO
 If yes, why is it necessary to breed animals at Dalhousie University?
2.
Who is responsible for managing the colony, weaning, record keeping, etc?
Name:
Phone / E-mail:
3.
Approximately how many female breeders will be needed in one year?
Do not include these numbers in Section 6b in Form A.
4.
Approximately how many male breeders will be needed in one year?
Do not include these numbers in Section 6b in Form A.
5.
Approximately how many offspring will be PRODUCED in one year?
Do not include these numbers in Section 6b in Form A.

How does this relate to your anticipated use?
6.
If more animals are produced than required for your experimental protocol(s), what happens
to the excess animals?
7.
Will the animals produced be used in protocols other than this one?
YES
NO
 If yes, list all the protocols:
8.
Will more than one investigator use the animals produced? YES
 If yes, list investigators:
NO
2015
9.
How often are breeding animals replaced and what criteria will be used for removing animals
from the colony?
10.
How will animals be identified?
Cage card
Ear punch
Microchip
11.
Tail tip
Other (describe and justify):
Anesthesia is required for tail tipping in animals older than 10 days. How old are the animals
when tail tips or other tissue are collected?


13.
(please describe)
Will animals produced require genotyping? YES
NO
 If yes, how is tissue collected for genetic characterization? Ear punch biopsies are
preferred for rodents and may be performed without anesthesia. Toe clipping is not
recommended and must be fully justified.
Ear punch
12.
Other
Will animals be anesthetized when tissue is collected? YES
NO
N/A
If yes, complete and include ATTACHMENT A (Anesthesia/Surgery)
Are these animals genetically modified? YES




NO
If yes, what is the name and function of the gene(s) that has been modified? How does
this modification relate to your study?
If yes, are there any known traits that will affect breeding and/or lifespan?
Describe any phenotypic abnormalities expected in these animals:
If these abnormalities will cause pain or distress, how will you minimize or alleviate it?