2012
ATTACHMENT F
Breeding Colonies
*Complete one ATTACHMENT F for EACH STRAIN you are breeding.
Note that you are required to track and report the number of animals produced by in-house breeding.
Investigator Name:
Species:
Strain Name:
Exact nomenclature:
1.
Are the animals required for use in this protocol available from a commercial supplier?
YES
NO
If yes, why is it necessary to breed animals at Dalhousie University?
(this answer section will expand)
2.
Who is responsible for managing the colony, weaning , record keeping etc?
Name:
Phone / E-mail:
3.
Approximately how many female breeders will be needed in one year?
4.
Approximately how many male breeders will be needed in one year?
5.
Approximately how many offspring will be PRODUCED in one year?
How does this relate to your anticipated use?
6.
If more animals are produced than required for your experimental protocol(s), what
happens to the excess animals?
7.
Will the animals produced be used in protocols other than this one?
YES
NO
If yes, list all the protocols:
8.
Will more than one investigator use the animals produced? YES
If yes, list investigators:
9.
How often are breeding animals replaced and what criteria will be used for removing
animals from the colony?
10.
How will animals be identified?
Cage card
Ear punch
Microchip
11.
Other
NO
(please describe):
Will animals produced require genotyping?
YES
NO
If yes, how is tissue collected for genetic characterization? Ear punch biopsies are
preferred for rodents and may be performed without anesthesia. Toe clipping is not
recommended and must be fully justified.
Ear punch
Tail tip
Other
(describe and justify):
(this answer section will expand)
2012
12.
13.
Anesthesia is required for tail tipping in animals older than 10 days. How old are the
animals when tail tips or other tissue are collected?
Will animals be anesthetized when tissue is collected?
YES
If yes, complete and include ATTACHMENT A (Anesthesia/Surgery)
NO
Are these animals genetically modified?
NO
YES
N/A
If yes, what is the name and function of the gene(s) that has been modified?
How does this modification relate to your study?
(this answer section will expand)
If yes, are there any known traits that will affect breeding and/or lifespan?
(this answer section will expand)
Describe any phenotypic abnormalities expected in these animals:
(this answer section will expand)
If these abnormalities will cause pain or distress, how will you minimize or alleviate it?
(this answer section will expand)