APPENDIX B: Breeding 1) Justify why animals need to be bred at UTEP. ☐ Animals are not available commercially ☐ Other: Click here to enter text. 2) Select breeding scheme. Select all that apply (Refer to UTEP IACUC Policy 013 Rodent Housing Densities & Overcrowding) ☐ ☐ ☐ ☐ Monogamous - 1 male and 1 female breeder per cage Pair or Trio - 1 male and no more than 2 female breeders per cage Other Harem – 3 females and 2 males OR 4 females and 1 male Continuous - Breeders kept together after litter is born to allow breeding during the immediate post-partum estrus ☐ Non-continuous - Breeding pair separated when female is visibly pregnant ☐ Other: Click here to enter text. 3) Identify personnel responsible for managing breeding colony and weaning of animals. 4) Number of breeders for 3 years. Be sure to include animal numbers on main protocol form under section C. Animal Requirements. Add rows if needed Species Strain # Male Breeders # Female Breeders Grand Total: 5) Estimated number of pups to be used/produced for 3 years. Add rows if needed. (Refer to UTEP IACUC Policy 016 Counting Fetal and Neonatal Rodents) Strain Estimated # needed for this protocol (as breeders or for other procedures) Estimated # to be transferred to another protocol Estimated # to be euthanized without use Total estimated # of pups 6) Disposition of Animals Page 1 of 2 Revised 2015 a) Breeders ONLY select all that apply ☐ Euthanize as described in protocol ☐ Transfer to another protocol. Explain: Click here to enter text. ☐ Use for experimental use. Briefly explain: Click here to enter text. b) Pups ONLY select all that apply ☐ Euthanize. Explain the method and why pups are being euthanized: Click here to enter text. ☐ Transfer to another protocol. Explain: Click here to enter text. ☐ Use for experimental procedures or breeders. Briefly explain: Click here to enter text. Page 2 of 2 Revised 2015