Office for Research Protections
__________________________________
The 330 Building, Suite 205
University Park, PA 16802
(814) 865-1775
Fax: (814) 863-8699
Request to add a New Strain to Animal Care and Use Protocol
****** ALL RESPONSES MUST BE TYPED ******
Once completed, this form can be sent via email to ORP-IACUC@rtto.psu.edu
Note: Emailed applications must be sent from the Principal Investigator (PI) directly, or the PI must be copied on the
email.
Principal Investigator:
Project Title:
IACUC #:
1. What is the source of the breeding animals?
2. Please provide the following information for each line.
2a. Provide the name or nomenclature used to identify each line. (Example: C.B17 – SCID)
as well as the strains phenotype.
Strain Name or Nomenclature
Phenotype (i.e. SCID Mouse = Severe Combined
Immuodeficient, T&B cell deficient)
2b. Is the phenotype of the breeders or offspring associated with physical abnormalities,
increased susceptibility to disease or a shortened lifespan.
NO
Affected Strain
YES, describe the problem and how the animals will be handled below;
Problem due to phenotype
How will the strain be handled?
2c. Do these animals require any special husbandry procedures?
NO
YES, please explain in the table below:
Strain requiring special
husbandry procedures
Husbandry procedures required
3. Is the new strain being added to a breeding colony?
YES, please complete the table below
Species
NO, go to Q5
No. of offspring to be Weaned
Animals (estimate)
No. of Breeders
Strain
4. Should your previous (i.e. already approved) estimate of total animals needed for this
breeding colony be increased by the number of breeders and offspring listed above (if you
already have enough animals approved for this colony, respond no):
YES
NO
5. Will samples be collected from the animals for genotyping? **
NO, go to Q6
YES, and will adhere to the IACUC approved procedures described below (*).
YES, but will deviate from the IACUC approved procedures described below
(*), please describe the procedures you will use.
* The sample should be as small as possible (1/2 cm or less should be adequate for most).
* Tail tips may be collected from mice that are less than 3 ½ weeks of age without
anesthesia.
* Animals older than 3 ½ weeks of age will be anesthetized with isoflurane for tail tip
amputation.
* As an alternative to tail snips, ear punches or an ear snip (2-3 mm snipped from the tip
of the ear)
may be collected from any age mouse without anesthesia.
**Please refer to IACUC Guideline II, Genotyping: Tail Clipping, Ear Punch, Ear Snip
6. Is the new strain being added to a research protocol?
YES, please complete the table below
Species
Strain
 NO
Number of additional (i.e. not already approved)
animals needed (if no additional animals are needed,
list zero):
7. If additional animals are needed to complete the research, please explain why the additional
animals are needed:
Click Here to Email This Application to ORP-IACUC@rtto.psu.edu
Note: Emailed applications must be sent from the Principal Investigator (PI) directly,
or the PI must be copied on the email.