Section B: Breeding Colony
Complete this form when breeding mice and rats to maintain experimental colonies. Submit
completed forms to iacuc@gwu.edu . To comply with NIH OBA guidelines, any transgenic
animal strain that is crossed to a wild type strain or a different transgenic strain requires
notification of the Institutional Biosafety Committee (IBC) before the breeding is initiated.
Please contact Dan Hibbing at rsodjh@gwu.edu with any questions about IBC review.
Date: [
]
Investigator: [
]
Contact Person (if other than PI): [
]
IACUC Protocol Number(s): [
]
Animal Holding Room Location: [
]
Contact Person and Phone Number for after hours/ weekend emergencies: [
Species: Mice
Strain(s):
1. [
2. [
3. [
4. [
5. [
Rats
Other: [
]
]
]
]
]
]
]
Source:
Jackson Labs
Other: [
Taconic
Harlan
NCI
Charles River
Hilltop
]
Catalog/strain identification number: [
]
If breeders are obtained from a source other than one of the approved commercial vendors listed
above, a health report must be reviewed and approved by the Attending Veterinarian before
animals will be allowed into the facility. Contact Dr. Kayleen Gloor at kayleen@.gwu.edu with
any questions.
Breeding Scheme: (Please describe in detail your breeding scheme – note: if harem breeding, pregnant females
MUST be removed to separate cages BEFORE they deliver)
Homozygous
Heterozygous
How many breeders are paired in one cage?
Males: [
]
Updated 9/2012 - AMM
Females:[
]
Section B: Breeding Colony
Are the females removed to separate cages to deliver and wean litters?
Yes
No
At what age are pups weaned? [
]
What is the expected average litter size?
What percentage of pups is expected to survive to weaning? [
]
At what age are breeders initially mated?
Males: [
]
Females: [
]
At what point are breeders retired, i.e., age, number of litters, etc.? [
]
What method of identification is used for individual animals?
Ear tag
Ear punch
Tattoo
None
Other: [
]
For transgenic strains, what testing is done to determine genotype, and at what age are the
animals tested? [
]
What percentage of pups is expected to be unusable for experiments due to incorrect
genotype, age, gender, or other factors? [
]
Describe the final disposition of any unusable animals, i.e., transfer to another PI,
euthanasia, etc. [
]
What additional strategies are required for poorly breeding strains: (e.g. light cycle,
enrichment, ensuring that animals from early litters are used as dams/sires, etc.) [
]
Quality control: For KO and transgenic strains what testing is done to ensure correct genotype
is maintained?
Copy of breeding card: (contact ARF (202-994-2871) – Marissa or Rob with help creating card, if needed).
Note that information on breeding cards should include:
o Investigator Name
o IACUC protocol number
o Cage #
o Dam and Sire identification & strain
o Date breeding pair established
o # of pups born and date of birth for each litter
o # of pups weaned of each sex and date of weaning
Updated 9/2012 - AMM