Custom Protein Expression Service Quote Request
Ⅰ Please fill in the form and send it to ji.lei@genecreate.com or contact with the skype jenny.lei17
ⅡWe will make an assessment according to your detailed information and send you the quotation as
soon as possible.
Ⅲ Thanks for your contact with GeneCreate, we will do our best to serve you. (www.genecreate.com)
Customer information
Contact person
Company Name
Tel
Email
Gene and protein information
Target protein
Gene/protein Name
Gen Bank / Uniprot ID:
email attachment)
Origin of Species
(or send us the protein amino acid sequence by
GeneCreate synthesis and optimize the gene
GeneCreate synthesis the gene without optimization）
Gene sources
Customers provide the expression plasmid
Customers provide non expression plasmid
Other
Please fill in the sequence information)
Expression
region
Vector
construction
GeneCreate construct expression vector
Customer provide the constructed expression vector
No need for mutation
Amino acid
mutation
Expression
system
Need for mutation
(If you have a requirement for the mutation, please provide the amino acid
sequence of the target protein.)
E.coli
Bacillus subtilis
Baculovirus - insect cells
Fusion tag
Tag
Protein
purification
The experiment
data
and information
provided
N-His
N-GST
N-SUMO
N-Myc
C-His
C-flag
No tag
Other tags
Cleave the tag
Yeast
Mammalian cell
N-MBP
C-HA
N-flag
N-HA
C-Myc
Don’t cleave the tag
Don’t purify the protein
Purity the protein(requirement
)
NOTE: If you have do ne the protein expression and purification by yourself, please
write the experiment result here.)
Service
Gene and Vector
Prokaryotic
expression
Gene synthesis
Plasmid construction
Service content
Express strain screening
Express strain screening
Inclusion body purification and renaturation
Deliverables
Strains or cell lines
Lysis Buffer
Protein
ml, concentration
ug/ul
mg
Other requirement
(for example the protein purity, concentration,
endotoxin, amplification etc.)
Yeast Expression
Service content
Transformation
Screening
Inducible expression of 10 strains
Inducible expression of 40 strains
Fermatation
Supernatant processing and one step purification
SDS-PAGE 及 WB identification
Deliverables
Strains or cell lines
Lysis Buffer
Protein
Baculovirus-Insect
cell expression
ml, concentration
ug/ul
mg
Other requirement
endotoxin, amplification etc.)
Service content
(for example the protein purity, concentration,
Construct the bacmid
Transfection and expression in small quantities
Fermentation
Deliverables
Purification
Strains or cell lines
Lysis Buffer
Protein
ml, concentration
ug/ul
mg
Other requirement
(for example the protein purity, concentration,
endotoxin, amplification etc.)
Mammalian cell
expression
Transfection type
Stable transfection
Transient transfection
Lentivirus Package
Service content
Cell strain
Transfection
Expression in small quantities
Fermatation
Deliverables
Purification
Strains or cell lines
Lysis Buffer
Protein
ml, concentration
ug/ul
mg
Other requirement
endotoxin, amplification etc.)
(for example the protein purity, concentration,
Project information
Project start time
Start at once
Within half a year
Within one month
Other time
Within two months