1. Please complete and email this form to bioprocess@genscript.com
and bioassay@genscript.com
2. Our Account Manager will contact you with a quote.
If you have registered an account with GenScript, you can identify yourself by giving us your name and email address or
Account No.
Name:
Account No.:
Phone:
Organization:
Shipping Address:
(Necessary to determine shipping cost)
Email Address:
What is the preferred gRNA-Cas9 delivery method?
Transfection-based Viral-based
Name of Target Gene: NCBI Accession Number (Gene ID):
DNA Sequence for Targeting
Does KO of the target gene affect cell growth?
Yes No Not sure
If yes, how to rescue the KO clones:____________________________________________________________
Does KO of the target gene affect cell survival?
Yes No Not sure
If yes, how to rescue the KO clones:____________________________________________________________
Name of Host Cell Line:
What is the suggested method for cell transfection (if transfection-based method has been chosen)?
Chemical transfection, please specify the reagent:_________________
Electroporation, please specify the program:_________________
Nucleofection, please specify the kit and program:_________________
Please provide transfection efficiency (if possible):____________________________________________
What is the preferred virus for gRNA-Cas9 delivery (if viral-based method has been chosen)?
Lentivirus AAV

What are the medium and additives for cell growth?
Medium:______________________________________________________
Additives:_____________________________________________________
Growth condition of host cell line? Adherent Suspension Both
Please provide cell doubling time:____________________________________
Resistance of the provided host cell line? G418 Puromycin Zeocin Hygromycin B
Blasticidin S Other__________ No resistance.
Can the cell line form single cell clones? Yes No Not sure
Will serial dilution affect cell growth rate? Yes No Not sure
Do you need GenScript to follow any special cell culture routine?
Yes, see below No
Please provide the protocol with information about the cell line and any special growth characteristics or requirements:
__________________________________________________________________________________________
Do the cells contain any human pathogen?
Yes, please specify:___________________________ No
Genotype of transgenic cell line:
Single allele Two alleles Multiple alleles (indicate number)______ All alleles (number unknown)
Please indicate the preferred type of analysis to characterize transgenic cell line (Extra fees may be charged):
Growth curve Western blot (antibodies provided by the customer) off-target analysis
What is the final application of the transgenic cell line?
Gene function analysis Assay development Drug screening Other
If Other, Please indicate your specific application and requirements:_________________________________
Comments:
Is this project for grant application purpose? Yes No
When will the project start?
Immediately Within one month Within three months Half a year later