GenCRISPR™ Custom Cell Line Development Service SC1652

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GenCRISPR™ Custom Cell Line Development Service SC1652 (Knock-out), SC1652-V (Knock-out) Instructions

1. Please complete and email this form to [email protected]

and [email protected]

2. Our Account Manager will contact you with a quote.

Customer Information

If you have registered an account with GenScript, you can identify yourself by giving us your name and email address or Account No.

Name: Account No.: Phone: Organization: Shipping Address:

(Necessary to determine shipping cost)

Email Address:

Genomic Editing

What is the preferred gRNA-Cas9 delivery method? Transfection-based Viral-based Name of Target Gene: NCBI Accession Number (Gene ID): DNA Sequence for Targeting Does KO of the target gene affect cell growth?

Yes No Not sure If yes, how to rescue the KO clones:____________________________________________________________

Does KO of the target gene affect cell survival?

Yes No Not sure If yes, how to rescue the KO clones:____________________________________________________________

Host Cell Line

Name of Host Cell Line: What is the suggested method for cell transfection (if transfection-based method has been chosen)?

Chemical transfection, please specify the reagent:_________________ Electroporation, please specify the program:_________________ Nucleofection, please specify the kit and program:_________________ Please provide transfection efficiency (if possible):____________________________________________

What is the preferred virus for gRNA-Cas9 delivery (if viral-based method has been chosen)?

Lentivirus AAV

What are the medium and additives for cell growth?

Medium:______________________________________________________ Additives:_____________________________________________________

Growth condition of host cell line?

Adherent Suspension Please provide cell doubling time:____________________________________

Resistance of the provided host cell line?

Blasticidin S Other__________ G418 Puromycin No resistance. Zeocin Both Hygromycin B

Can the cell line form single cell clones?

Yes No Not sure

Will serial dilution affect cell growth rate?

Yes No Not sure

Do you need GenScript to follow any special cell culture routine?

Yes, see below No Please provide the protocol with information about the cell line and any special growth characteristics or requirements: __________________________________________________________________________________________

Do the cells contain any human pathogen?

Yes, please specify:___________________________

Requirements for Customized Transgenic Cell Line:

Genotype of transgenic cell line:

No

Single allele Two alleles Multiple alleles (indicate number)______ All alleles (number unknown) Please indicate the preferred type of analysis to characterize transgenic cell line (Extra fees may be charged):

Growth curve Western blot (antibodies provided by the customer) off-target analysis

What is the final application of the transgenic cell line?

Gene function analysis Assay development Drug screening Other If Other, Please indicate your specific application and requirements:_________________________________

Comments:

Project Information

Is this project for grant application purpose? When will the project start?

Immediately Within one month Yes Within three months No Half a year later

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