Transient & Stable Cell Line Quotation Inquiry Form Instructions Please answer as many questions as possible and send the completed form to bioprocess@genscript.com. You can also fax it to 1-732-210-0262 or 1-732-885-5878. Assuming all required information has been provided, our service representative will get back to you with a free quote within 1 business day. Customer Information If you have registered an account with GenScript, you can identify yourself by giving us your name and email address or the Account No. Name Account No. Phone Organization Shipping Address (necessary to Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. Click here to enter text. determine shipping cost) Email Address Intended Service Target Gene/Protein The native form of this protein is (select all that apply) Starting material Preferred host Stability test Screening method for stable clones GLP-compliant service Click here to enter text. ☐ Transient Expression ☐ Stable Cell Line Development ☐ No Preference Name Click here to enter text.Species Click here to enter text.MW (kDa)Click here to enter text.Sequence Click here to enter text. Protein sequence is crucial for evaluation and gene synthesis. Please provide sequence information when you submit this form ☐ Ab or Ab fragment ☐ Cytoplasmic ☐ Enzyme ☐ Membrane-bound ☐ Nuclear Protein ☐ Protease ☐ Secreted ☐ Toxic ☐ Other If “Other”, please specify Click here to enter text. ☐ Gene synthesis with codon optimization (recommended) ☐ Gene synthesis without codon optimization ☐ I will provide DNA template ☐ I will provide expression ready construct If submitting samples, please mail them together with a hard copy of the completed Quotation/order form to “Protein Services, GenScript, 860 Centennial Ave., Piscataway, NJ 08854, USA” ☐ No preference ☐ HEK293 ☐ CHO-K1 ☐ Other If “Other”, please specify Click here to enter text. ☐ Yes ☐ No ☐ ELISA ☐ Flow cytometry ☐ qPCR ☐ Western Blot ☐ Other If “Other”, please specify Click here to enter text. ☐ Yes ☐ No Selectable marker Epitope tag selection Purification methods Characterization (you can choose more than one) Intended Applications (select all that apply) Has gene been tested for stable expression previously? Has gene been tested for transient expression previously? Approximate timeline for project initiation Comments ☐ Blasticidin ☐ Neomycin ☐ Puromycin ☐ GFP-Blasticidin ☐ RFP-Blasticidin ☐ GFP-Puromycin ☐ RFP-Puromycin ☐ His ☐HA ☐ Myc ☐ V5 ☐ GFP ☐ RFP ☐ CFP ☐ None ☐ Other In case of “Other”, please specify Click here to enter text. Add tag(s) to the ☐ N-terminus ☐ C-terminus ☐ Other If “Other”, please specify Click here to enter text. Use GenScript’s proprietary purification protocol? (recommended) ☐ Yes ☐ No, I have custom protocol (please provide during submission) ☐ Other If “Other”, please specify Click here to enter text. ☐ Endotoxin ☐ ELISA ☐ HPLC ☐ IEF ☐ MALDI-TOF ☐ N-terminal sequencing ☐ SDS-PAGE ☐ Western Blot ☐ Other If “Other”, please specify Click here to enter text. ☐ Activity assay ☐ Antigen ☐ HTS ☐ In vivo studies ☐ Protein-Protein interactions ☐ NMR ☐ X-ray Crystallography ☐ Other Click here to enter text. If “Other”, please specify Click here to enter text. ☐ Yes ☐ No ☐ Unsure Enter comments if any Click here to enter text. ☐ Yes ☐ No ☐ Unsure Enter comments if any Click here to enter text. ☐ ASAP ☐ Within 1 month ☐ Within 3 months Click here to enter text. Please enter any comments in this field ☐ Within 6 months