Q) Define
1. cell line
2. Balanced salt solution
3. Primary cell culture
4. Transfection
5. Expression vectors
6. Artificial insemination
7. Hybridoma technology
8. serum free media
9. artificial skin
10. gene therapy
Q) what are physical requirements of growing animal cells in culture?
Q) What are various sterilization techniques used in animal cell culture?
Q) what is subculture and what is criteria of subculture? Write a short note on suspension culture?
Q) Write a short note on growth curve of animal cells in culture.
Q) Explain in detail characterization of cell lines.
Q) what are the methods of Isolation, disaggregation and culture of animal cells.
Q) Explain different methods of organ culture along with its advantages and limitations.
Q) Explain in detail hybridoma technology and HAT selection.
Q) Give various benefits we mat extract from transgenic animals.
Q) Explain the methodology and applications of transgenic mice.
Q) Explain in detail different gene delievery methods and which methods are most useful?
Q) What is gene therapy? Howw it is done? What is its current status?
Q) Explain in deatail various mathods of embyo transfer along with its advantages and limitations.
Q) How a protein can be overexpressed using expression vectors?
Q) What are selectable markers? Give various types?
Q) what are superbugs? Give detail about antibiotic resistance of animal cells.