E. coli BacPowerTM Guaranteed Package Quotation Form Instructions Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the E. coli BacPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly. Customer Information If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No. Name: Account No.: Phone: Organization: Shipping address: (Necessary to determine the shipping cost) Email address: Project Information Nature of inquiry: Order initiation: Quote for ordering Immediately For pricing estimation Within three months For grant application Beyond three months Please read the following terms and conditions for the BacPower TM Guaranteed Package: Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for membrane proteins and toxic proteins. For eligible proteins, a minimum of 3 mg soluble protein with >75% purity are guaranteed. The protein service will be free of charge if the expression level is too low to eventually obtain 3 mg of purified protein after GenScript's evaluation. To increase the expression level of your protein, optimized genes are recommended using our OptimumGeneTM technology (note: gene synthesis is at additional cost). Expressed insoluble proteins will be subject to refolding using our FoldArt TM technology at no additional fee. Price structure for the BacPowerTM Guaranteed Package: Protein purity 75% purity 85% purity Protein tag With tag Tag-free With tag Tag-free Starting Price for 3mg protein $1980 (3mg) $3700 (3mg) $2340 (3mg) $ 4060 (3mg) Additional amount of protein Additional protein for $198 per 1mg Additional protein for $370 per 1mg Additional protein for $234 per 1mg Additional protein for $406 per 1mg Protein Expression & Purification Protein name: Species: MW: Protein sequence (crucial for evaluation) or accession number: Note: Please enter the protein sequence if mutants are included in your protein sequence. Gene synthesis with codon optimization (Starting from $0.35/bp) (Please enter your gene sequence: ) Gene synthesis according to natural gene sequence (Codon optimized gene is strongly recommended for protein expression) (Please enter your gene sequence: ) Starting material: Your own template DNA (Codon optimized gene is strongly recommended for protein expression) (Please attach vector map (e.g. NTI file), and enter your vector name: , and gene sequence: Note: For natural gene sequence or template from the client, GenScript need to evaluate the codon usage and only guarantee those projects pass the evaluation. Cytoplasmic protein Membrane-bound protein Toxic to E. coli IgG antibody Other (please specify: ) Protein properties: Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized protein service. Protein amount: 3mg; Protein purity: 75% purity; Protein Tag Tag-free; Protein characterization: Tag information: Other comments: Other amount (please specify: ) 85% purity With tag (If with tag, please specify it in the last three rows of the table) SDS-PAGE & Western blot HPLC MS SEC MALDI-TOF ELISA N-terminal sequence Note: SDS-PAGE & Western blot is FREE of charge; The other experiments are at additional cost. For tag-free proteins, GenScript will perform MOLDI-TOF for FREE for the quality control purpose. GenScript reserves the right to choose the best tag and tag position in order to guarantee the target protein expression.