InsectPowerTM Guaranteed Package Quotation Form Instructions Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the InsectPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly. Customer Information If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No. Name: Account No.: Phone: Organization: Shipping address: (Necessary to determine the shipping cost) Email address: Project Information Nature of inquiry: Quote for ordering For pricing estimation For grant application Order initiation: Immediately Within three months Beyond three months Please read the following terms and conditions for the InsectPowerTM Guaranteed Package: Terms and Conditions: 1. Protein sequences are subject to pre-project analysis. Exclusions are applicable for some requests: Ⅰ: large genes, longer than 3 kbp or high molecular-weight protein, larger than 110 kDa; Ⅱ: Membrane or toxic proteins; Ⅲ: High purity-proteins, more than 85% purity; Ⅳ: The customers provide the expression construct/virus/Bacmid DNA. 2. The protein service will be free of charge if the expression level is too low to eventually obtain 1 mg of purified protein after GenScript’s evaluation. 3. Up to 2 fusion tag(s) may be tested to increase the expression level and solubility of the protein and applied for protein production and purification. 4. Gp67 signal peptide may be added for extracellular expression and residued after purification. 5. GenScript reserves the right to choose the best tag and tag position in order to guarantee the target protein expression. 6. For eligible proteins, a minimum of 1 mg soluble protein with 75% purity will be delivered to customers Price structure for the InsectPowerTM Guaranteed Package: Protein purity Protein tag Promotional price Additional amount of protein 75% purity With tag Starting from $3950 (1 mg) Quote Protein Expression & Purification Please fill the form carefully, and asterisk (*) indicates required fields. Protein name: *Protein Species: MW: Protein sequence (crucial for evaluation) or accession number: Information Note: Please enter the protein sequence if mutants are included in your protein sequence. Gene synthesis with codon optimization (FREE of charge, Codon optimized gene is strongly recommended for protein expression) (Please enter your gene sequence: ) *Starting Material: Gene synthesis according to natural gene sequence (Please enter your gene sequence: ) Note: For natural gene sequence from the client, GenScript need to evaluate the codon usage and only guarantee those projects pass the evaluation. Protein Properties: Cytoplasmic protein Membrane-bound protein IgG antibody Other (please specify: ) Toxic to Insect cells Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized protein service. Protein Amount: Protein Purity: Protein 1mg; Other amount (please specify: ) 75% purity; Other purity (please specify: ) SDS-PAGE & Western blot SEC ELISA HPLC MALDI-TOF N-terminal sequence MS Characterization : Note: SDS-PAGE & Western blot is FREE of charge and strongly recommended; The other experiments are offered at additional cost. Do you want GenScript to add the tag(s) at the N-terminus? Tag position: Yes No, I have designated place(s) to add the tag(s) (Please specify: ) Note: Customer selected tag position will be the first choice. GenScript reserves the right to choose the tag position in order to guarantee the target protein expression. Please select one of your preferred tag to facilitate the expression and purification of your target protein: 6xHis Tag selection: 8xHis 10xHis GST MBP Fc Strep II Flag Other (please specify: ) Note: A customer selected tag or small tag (e.g. -6xHis) will be the first choice for protein production. GenScript reserves the right to choose a best tag in order to guarantee the target protein expression. Other Comments: