Custom Protein Expression Service Quote Request Please fill in the form and return it either via e-mail to: sales@2BScientific.com or Fax: +44 (0)1869238034 We will make an assessment according to your detailed information and send you the quotation as soon as possible. Customer information Contact Tel Target protein Institute Email Gene/protein Name Gen Bank / Uniprot ID: attachment) Origin of Species (or send us the protein amino acid sequence by email GeneCreate synthesis and optimize the gene GeneCreate synthesis the gene without optimization) Gene sources Customers provide the expression plasmid Customers provide non expression plasmid Other Please fill in the sequence information) Expression region Vector construction GeneCreate construct expression vector Customer provide the constructed expression vector No need for mutation Amino acid mutation Expression system Need for mutation (If you have a requirement for the mutation, please provide the amino acid sequence of the target protein.) E.coli Bacillus subtilis Baculovirus - insect cells Fusion tag Tag Protein purification The experiment data and information provided N-His N-GST N-SUMO N-Myc C-His C-flag No tag Other tags Cleave the tag Yeast Mammalian cell N-MBP C-HA N-flag N-HA C-Myc Don’t cleave the tag Don’t purify the protein Purity the protein(requirement ) NOTE: If you have do ne the protein expression and purification by yourself, please write the experiment result here.) Service Gene and Vector Prokaryotic expression Gene synthesis Plasmid construction Service content Express strain screening Express strain screening Inclusion body purification and renaturation Deliverables Strains or cell lines Lysis Buffer Protein ml, concentration ug/ul mg Other requirement (for example the protein purity, concentration, endotoxin, amplification etc.) Yeast Expression Service content Transformation Screening Inducible expression of 10 strains Inducible expression of 40 strains Fermatation Supernatant processing and one step purification SDS-PAGE WB identification Deliverables Strains or cell lines Lysis Buffer Protein Baculovirus-Insect cell expression ml, concentration ug/ul mg Other requirement amplification etc.) Service content (for example the protein purity, concentration, endotoxin, Construct the bacmid Transfection and expression in small quantities Fermentation Deliverables Purification Strains or cell lines Lysis Buffer Protein Mammalian cell expression ml, concentration ug/ul mg Other requirement amplification etc.) Transfection type Stable transfection (for example the protein purity, concentration, endotoxin, Transient transfection Lentivirus Package Service content Cell strain Transfection Expression in small quantities Fermatation Deliverables Purification Strains or cell lines Lysis Buffer Protein ml, concentration ug/ul mg Other requirement amplification etc.) (for example the protein purity, concentration, endotoxin, Project information Project start time Start at once Within half a year Within one month Other time Within two months