Supplementary Material for Estimation of Black-tailed Prairie Dog Colonies in Montana RYAN L. RAUSCHER,1,2 Montana Fish, Wildlife and Parks, 54078 United States Highway 2 W, Glasgow, MT 59230, USA SCOTT J. STORY, Montana Fish, Wildlife and Parks, P.O. Box 200701, Helena, MT 59620, USA JUSTIN A. GUDE, Montana Fish, Wildlife and Parks, P.O. Box 200701, Helena, MT 59620, USA ROBIN E. RUSSELL,3 Montana Fish Wildlife and Parks, 1400 19th Street, Bozeman, MT 59717, USA 1 E-mail: rrauscher@mt.gov 2 Present address: Montana Fish, Wildlife & Parks, 514 S Front Street, Suite C, Conrad, MT 59425, USA 2 Present address: United States Geological Survey National Wildlife Health Center, Madison, WI 53711, USA Appendix The following is derived from White et al. (2005) and Odell et al. (2008) and describes the modeling approach used in the manuscript. Estimates for correcting colony lengths measured from the air (CLA) for each colony i were calculated by assuming CLA (y_lengthi) were normally distributed with error and that colony lengths on the ground (CLG) represented the true lengths (z_lengthi). y _ lengthi ~ dnorm (µi , τ); where τ 1/ σ2 , the prior on σ ~ uniform 0,10 , and µi z _ lengthi β. Here τ represents the precision parameter, and β is the estimate of the ratio of true length to estimated length from the air. The inverse of the estimates of β were used as a correction factor for all y_lengthi to estimate z_lengthi. Then corrected lengths were summed for each individual transect, and the sum was divided by the transect length to calculate the ratio (rt) of active colony to surveyed transect for each transect t (Odell et al. 2008). Mean ratios, μ_ratios, for each strata s were calculated by averaging rt within a strata. To correct for the misidentification of inactive colonies as active from the air, we assumed that the designation of ‘active’ or ‘inactive’ on the ground represented the true state of the colony (za) and za ~ Bern(ψ), where the prior on ψ ~ Beta(1,1) and is the probability that a colony is active. This represents the latent or unobserved probability that a given colony transect is active. The observed classifications (ya) are Bernoulli-distributed ya ~ Bern(pi), where pi is the probability of classifying a colony as active. This formulation represents the observation process; that is, the probability of identifying a colony as active versus the probability the colony truly is active (ψ). pi = za + cspec ×(1 − za); with a prior on cspec ~ Beta(1,1,) equals 1-specificity, or the false positive rate (the proportion of inactive colonies that were classified as active from the air). Therefore the probability of identifying a colony as active from the air is equal to 1 if the colony is active on the ground (there were no active colonies identified as inactive from the air; i.e., no false positives), and equal to cspec, the false positive rate, if the colony was not active on the ground. To calculate the proportion of occupied and unoccupied area for each stratum, we first calculated the proportion of active and inactive towns on each transect (rt and ri). Then we calculated the mean rt and ri for each stratum (county), μ_ratios. Finally, we multiplied the area of each stratum by the stratum-specific ratio, μ_ratios, to determine the total area of active and inactive colonies in each stratum.