I had a straight through long haul fiber connection from my 6509 to a 3750 provided by Charter. That has changed now to a ATT OPT-E-MAN fiber connection using their Cisco ME3400 switches on both ends with twist pair cable connections from them to my 6509 and to a 3560 on the remote end. The new fiber connection comes into the remote building where we had a 3560 that has fiber connection to the 3750 used for the Charter fiber connection. With the new fiber connection not all the devices on the 3750 are able to find the gateway IP address 10.4.1.254 that I have set on the 6509. All the devices on the 3560 that is directly attached to the Cisco ME3400 work. I have reset the 3750 and 3560 and the computers that are not working. When I switch back to the old Charter straight through fiber connection everything works. The next time I disconnect the old Charter fiber connection from the 3750 and connect the new fiber to the 3560 different computer are not working now and other computers that were working are now working. I am not able to ping the gateway 10.4.1.254 that is on the 6509 from the computers not working when on the new fiber connection. Old Fiber connection 6509---long haul fiber------3750--- Multi Mode fiber ---3560 New Fiber connection 6509---TP cable---ME3400-----fiber------ME3400----TP cable----3560---fiber----3750 Old Charter Fiber Configuration working 6509 fiber connection to 3750 interface GigabitEthernet5/15 switchport switchport access vlan 4 switchport trunk encapsulation isl switchport trunk allowed vlan 1-4,7,9,11,15 switchport mode trunk spanning-tree bpdufilter enable spanning-tree bpduguard enable interface Vlan4 on 6509 serves as the gateway for VLAN 4 computers ip address 10.4.1.254 255.255.0.0 ip helper-address 10.3.1.8 ip helper-address 10.3.1.9 ip pim dense-mode ip route-cache flow 3750 fiber connection to 6509 interface GigabitEthernet1/0/52 switchport trunk encapsulation isl switchport mode trunk interface Vlan1 ip address 10.11.2.1 255.255.0.0 no ip route-cache ! interface Vlan4 no ip address no ip route-cache ! ip default-gateway 10.11.1.254 Ports 1-48 on 3750 configured the same switchport access vlan 4 switchport mode access spanning-tree portfast New ATT fiber configuration not working 6509 CAT5 TP connection to ATT OPT-e-MAN ME3400 switch interface GigabitEthernet7/38 no keepalive speed 100 duplex full switchport switchport access vlan 4 switchport trunk allowed vlan 1-4,7,9,11 switchport mode trunk 3560 CAT5 TP connection to ATT OPT-e-MAN ME3400 switch interface FastEthernet0/24 description Court House 6509 switchport access vlan 4 switchport trunk encapsulation dot1q switchport mode trunk switchport nonegotiate no keepalive duplex full speed 100 ! 3560 fiber connection to 3750 interface GigabitEthernet0/1 switchport trunk encapsulation dot1q switchport mode trunk rmon collection stats 11 owner monitor ! interface Vlan1 ip address 10.11.4.1 255.255.0.0 ! interface Vlan4 no ip address ip helper-address 10.3.1.8 ip helper-address 10.3.1.9 ! ip default-gateway 10.11.1.254 Ports 1-23 on 3560 configured the same switchport access vlan 4 switchport mode access spanning-tree portfast