E. coli BacPowerTM Guaranteed Package Quotation Form
Instructions
Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the E. coli
BacPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly.
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email
address or Account No.
Name:
Account No.:
Phone:
Organization:
Shipping address:
(Necessary to determine
the shipping cost)
Email address:
Project Information
Nature of inquiry:
Order initiation:
Quote for ordering
Immediately
For pricing estimation
Within three months
For grant application
Beyond three months
Please read the following terms and conditions for the BacPower TM Guaranteed Package:
Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for
membrane proteins and toxic proteins. For eligible proteins, a minimum of 3mg soluble protein with 75% or 85% purity
will be delivered to customers. The protein services will be free of charge if GenScript's evaluation reveals that the
expression level is insufficient to eventually obtain 3mg of purified protein. To increase the expression level of your
protein, gene optimization using our OptimumGeneTm technology is recommended. Up to 3 fusion tag(s) may be
tested to increase the expression level and solubility of the protein and are applied for protein production and
purification. Fusion tags may be removed by introducing an optional protease cleavage site between the tag and the
target protein (note: tag removal service is offered at extra cost) . Insoluble proteins will be subject to refolding using
our FoldArtTm technology at no additional fee.
* Guaranteed services only apply to proteins with molecular mass no more than 100KDa.
Price structure for the BacPowerTM Guaranteed Package:
Protein purity
75% purity
85% purity
Protein tag
With tag
Tag-free
With tag
Tag-free
Promotional price
Starting from $2200 (3mg)
Starting from $3700 (3mg)
Starting from $2560 (3mg)
Starting from $4060 (3mg)
Note
DNA cloning fee is FREE
DNA cloning fee is FREE
DNA cloning fee is FREE
DNA cloning fee is FREE
Protein Expression & Purification
Protein name:
Species:
MW:
Protein sequence (crucial for evaluation) or accession number:
Note: Please enter the protein sequence if mutants are included in your protein sequence.
Gene synthesis with codon optimization (FTEE of charge)
(Please enter your gene sequence: )
Gene synthesis according to natural gene sequence (Codon optimized gene is strongly
recommended for protein expression)
(Please enter your gene sequence: )
Starting Material:
Your own template DNA (Codon optimized gene is strongly recommended for protein expression)
(Please attach vector map (e.g. NTI file), and enter your vector name:
, and gene
sequence:
Note: For natural gene sequence or template from the client, GenScript need to evaluate the codon
usage and only guarantee those projects pass the evaluation.
Cytoplasmic protein
Membrane-bound protein
Toxic to E. coli
IgG antibody
Other (please specify: )
Protein Properties:
Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized
protein service.
Protein Amount:
3mg;
Protein Purity:
75% purity;
Protein Tag
Tag-free;
Protein
Characterization:
Tag Information:
Other Comments:
Other amount (please specify: )
85% purity
With tag (If with tag, please specify it in the last three rows of the table)
SDS-PAGE & Western blot
HPLC
MS
SEC
MALDI-TOF
ELISA
N-terminal sequence
Note: SDS-PAGE & Western blot is FREE of charge; The other experiments are at additional cost. For
tag-free proteins, GenScript will perform MOLDI-TOF for FREE for the quality control purpose.
GenScript reserves the right to choose the best tag and tag position in order to guarantee the target
protein expression.