E. coli BacPowerTM Guaranteed Package Quotation Form Instructions Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the E. coli BacPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly. Customer Information If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No. Name: Account No.: Phone: Organization: Shipping address: (Necessary to determine the shipping cost) Email address: Project Information Nature of inquiry: Order initiation: Quote for ordering Immediately For pricing estimation Within three months For grant application Beyond three months Please read the following terms and conditions for the BacPower TM Guaranteed Package: Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for membrane proteins and toxic proteins. For eligible proteins, a minimum of 3 mg soluble protein with >75% purity are guaranteed. The protein service will be free of charge if the expression level is too low to eventually obtain 3 mg of purified protein after GenScript's evaluation. To increase the expression level of your protein, optimized genes are recommended using our OptimumGeneTM technology (note: gene synthesis is at additional cost). Expressed insoluble proteins will be subject to refolding using our FoldArtTM technology at no additional fee. Price structure for the BacPowerTM Guaranteed Package: Protein purity 75% purity 85% purity Protein tag With tag Tag-free With tag Tag-free Starting Price for 3mg protein $1980 (3mg) $3700 (3mg) $2340 (3mg) $ 4060 (3mg) Additional amount of protein Additional protein for $198 per 1mg Additional protein for $370 per 1mg Additional protein for $234 per 1mg Additional protein for $406 per 1mg Protein Expression & Purification Protein name: Species: MW: Protein sequence (crucial for evaluation) or accession number: Note: Please enter the protein sequence if mutants are included in your protein sequence. Gene synthesis with codon optimization (Starting from $0.35/bp) (Please enter your gene sequence: ) Gene synthesis according to natural gene sequence (Codon optimized gene is strongly recommended for protein expression) (Please enter your gene sequence: ) Starting material: Your own template DNA (Codon optimized gene is strongly recommended for protein expression) (Please attach vector map (e.g. NTI file), and enter your vector name: , and gene sequence: Note: For natural gene sequence or template from the client, GenScript need to evaluate the codon usage and only guarantee those projects pass the evaluation. Cytoplasmic protein Membrane-bound protein Toxic to E. coli IgG antibody Other (please specify: ) Protein properties: Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized protein service. Protein amount: 3mg; Protein purity: 75% purity; Protein Tag Tag-free; Protein characterization: Tag position: Other amount (please specify: ) 85% purity With tag (If with tag, please specify it in the last three rows of the table) SDS-PAGE & Western blot HPLC MS SEC MALDI-TOF ELISA N-terminal sequence Note: SDS-PAGE & Western blot is FREE of charge; The other experiments are at additional cost. For tag-free proteins, GenScript will perform MOLDI-TOF for FREE for the quality control purpose. Do you want GenScript to add the tag(s) at the N-terminus? Yes No, I have designated place(s) to add the tag(s) (Please specify: ) Note: Customer selected tag position will be the first choice. GenScript reserves the right to choose the tag position in order to guarantee the target protein expression. Please select up to three tags to facilitate the expression and purification of your target protein: Tag selection: 6xHis GST Other (please specify: ) MBP Trx TF Sumo Note: A customer selected tag or small tag (e.g. -6xHis) will be the first choice for protein production. GenScript reserves the right to choose a best tag in order to guarantee the target protein expression. Other comments: