Protein Expression & Purification

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E. coli BacPowerTM Guaranteed Package Quotation Form
Instructions
Please answer all of the following questions and send the completed form to protein@genscript.com if you choose the E. coli
BacPowerTM Guaranteed Package. Our service representative will contact you with a quote shortly.
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email
address or Account No.
Name:
Account No.:
Phone:
Organization:
Shipping address:
(Necessary to determine
the shipping cost)
Email address:
Project Information
Nature of inquiry:
Order initiation:
Quote for ordering
Immediately
For pricing estimation
Within three months
For grant application
Beyond three months
Please read the following terms and conditions for the BacPower TM Guaranteed Package:
Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for
membrane proteins and toxic proteins. For eligible proteins, a minimum of 3 mg soluble protein with >75% purity
are guaranteed. The protein service will be free of charge if the expression level is too low to eventually obtain 3
mg of purified protein after GenScript's evaluation. To increase the expression level of your protein, optimized
genes are recommended using our OptimumGeneTM technology (note: gene synthesis is at additional cost).
Expressed insoluble proteins will be subject to refolding using our FoldArtTM technology at no additional fee.
Price structure for the BacPowerTM Guaranteed Package:
Protein purity
75% purity
85% purity
Protein tag
With tag
Tag-free
With tag
Tag-free
Starting Price for
3mg protein
$1980 (3mg)
$3700 (3mg)
$2340 (3mg)
$ 4060 (3mg)
Additional amount of protein
Additional protein for $198 per 1mg
Additional protein for $370 per 1mg
Additional protein for $234 per 1mg
Additional protein for $406 per 1mg
Protein Expression & Purification
Protein name:
Species:
MW:
Protein sequence (crucial for evaluation) or accession number:
Note: Please enter the protein sequence if mutants are included in your protein sequence.
Gene synthesis with codon optimization (Starting from $0.35/bp)
(Please enter your gene sequence: )
Gene synthesis according to natural gene sequence (Codon optimized gene is strongly
recommended for protein expression)
(Please enter your gene sequence: )
Starting material:
Your own template DNA (Codon optimized gene is strongly recommended for protein expression)
(Please attach vector map (e.g. NTI file), and enter your vector name:
, and gene
sequence:
Note: For natural gene sequence or template from the client, GenScript need to evaluate the codon
usage and only guarantee those projects pass the evaluation.
Cytoplasmic protein
Membrane-bound protein
Toxic to E. coli
IgG antibody
Other (please specify: )
Protein properties:
Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized
protein service.
Protein amount:
3mg;
Protein purity:
75% purity;
Protein Tag
Tag-free;
Protein
characterization:
Tag position:
Other amount (please specify: )
85% purity
With tag (If with tag, please specify it in the last three rows of the table)
SDS-PAGE & Western blot
HPLC
MS
SEC
MALDI-TOF
ELISA
N-terminal sequence
Note: SDS-PAGE & Western blot is FREE of charge; The other experiments are at additional cost. For
tag-free proteins, GenScript will perform MOLDI-TOF for FREE for the quality control purpose.
Do you want GenScript to add the tag(s) at the N-terminus?
Yes
No, I have designated place(s) to add the tag(s) (Please specify: )
Note: Customer selected tag position will be the first choice. GenScript reserves the right to choose the
tag position in order to guarantee the target protein expression.
Please select up to three tags to facilitate the expression and purification of your target protein:
Tag selection:
6xHis
GST
Other (please specify: )
MBP
Trx
TF
Sumo
Note: A customer selected tag or small tag (e.g. -6xHis) will be the first choice for protein production.
GenScript reserves the right to choose a best tag in order to guarantee the target protein expression.
Other comments:
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