E. coliBacPowerTM Guaranteed Package Quotation Form Instructions Please answer all of the following questions and send the completed form to protein@genscript.com.Our service representative will contact you with a quote shortly. Customer Information If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No. Name: Account No.: Phone: Organization: Shipping address: (Necessary to determine the shipping cost) Email address: Project Information Nature of inquiry: Order initiation: Quote for ordering Immediately For pricing estimation For grant application Within three months Beyond three months Please read the following terms and conditions for the BacPowerTMGuaranteed Package: Terms and Conditions: Protein sequences are subject to pre-project analysis. Exclusions are applicable for membrane proteins and toxic proteins. For eligible proteins, a minimum of 3 mg soluble protein with 75% or 85% purity will be delivered to customers. The protein services will be free of charge if GenScript's evaluation reveals that the expression level is insufficient to eventually obtain 3mg of purified protein. To increase the expression level of your protein, gene optimization using our OptimumGeneTm technology is recommended. Up to 3 fusion tag(s) may be tested to increase the expression level and solubility of the protein and are applied for protein production and purification. Fusion tags may be removed by introducing an optional protease cleavage site between the tag and the target protein (note: tag removal service is offered at extra cost). Insoluble proteins will be subject to refolding using our FoldArtTm technology at no additional fee. *Guaranteed services only apply to proteins with molecular mass no more than 100KDa. Price structure for theBacPowerTMGuaranteed Package: Protein purity 75% purity 85% purity Protein tag With tag Tag-free With tag Tag-free Protein Expression& Purification Promotional price Starting from $2200 (3mg) Starting from $3700 (3mg) Starting from $2560 (3mg) Starting from $4060 (3mg) Note DNA cloning fee is FREE DNA cloning fee is FREE DNA cloning fee is FREE DNA cloning fee is FREE Protein name: Species: MW: Protein sequence (crucial for evaluation) or accession number: Note: Please enter the protein sequence if mutants are included in your protein sequence. Gene synthesis with codon optimization (FREE of charge) (Please enter your gene sequence: ) Gene synthesis according to natural gene sequence (Codon optimized gene is strongly recommended for protein expression) (Please enter your gene sequence: ) Starting Material: Protein Properties: Your own template DNA (Codon optimized gene is strongly recommended for protein expression) (Please attach vector map (e.g. NTI file), and enter your vector name: , and gene sequence: Note:When a natural gene sequence or template is provided by the client, GenScript needs to evaluate the codon usage and only guarantees those projects which pass the evaluation. Cytoplasmic protein Membrane-bound protein Toxic to E. coli IgG antibody Other (please specify: ) Note: If the target protein is membrane-bound or toxic to the bacterial host, please quote customized protein service. Protein Amount: 3mg; Other amount (please specify: ) ProteinPurity: 75% purity; Protein Tag Tag-free; 85% purity With tag (If with tag, please specify it in the Tag Information) SDS-PAGE & Western blot HPLC MS Protein Characterization: SEC MALDI-TOF ELISA N-terminal sequence Note: SDS-PAGE & Western blot is FREE of charge and strongly recommended;The other experimentsare offered at additional cost. For tag-free proteins, GenScript will perform MALDI-TOF for FREE for quality control purposes. Your preferred tag: Tag Information: GenScriptwill prioritize your preferred tag as the 1st choice, but we reserve the right to choose the best tag and tag position in order to guarantee the target protein expression.If you prefer to use a specific tag, please choose our BacPowerTMCustomized Protein Service (SC1318) as an alternative. Other Comments: