Capillary Electrophoresis SOP
*Turn on and let warm up for 30 minutes. (There is a button on the front of the instrument)
Set up:




Prepare buffer and samples in 1.5mL glass vials
Using a P1000 pipette add about 1.4mL (pipette 700mL two times) of Run Buffer A, Solution A
(0.1M NaOH), Distilled Water, and 1.0M HCL
Place cap on vials and take note of where they are placed in the loading tray.
o In another vial, add about 1.0mL of Text Mixture B and place cap on
On the computer: click direct control panel and then load
o Wait until the buffer trays are brought forward and then open the transparent cover.
o Insert buffer vials to the front left inlet buffer tray (BI)
o Place another vial with Run Buffer A in the outlet buffer tray (BO)
o Place sample in sample inlet tray (SI)
o Place empty waste vial in sample outlet tray (SO)
o Record final position of vials in notebook
Running the sample:




On computer, build method for running the sample based on following conditions
DO NOT CHANGE THE INITIAL CONDITION
PDA detector initial condition: use channel 214nm
Time program:
o Rinse 1 min (20 psi, forward pressure) from 1.0M HCl to waste
o Rinse 1 min (20 psi, forward pressure) from DI water to waste
o Rinse 2 mins (20 psi, forward pressure) from 0.1M NaOH to waste
o Rinse 1 min (20 psi, forward pressure) from DI water to waste
o Rinse 2 mins (20 psi, forward pressure) from Run Buffer A to waste
o Inject (0.5 psi pressure, 10 seconds) from sample vial (text mixture B) to waste
o Separate at 0.0 min (Duration: 7 mins, Voltage: 25 KV, Ramp: 0.17 min) from Run Buffer
A to Run Buffer A
o Autozero at time 1.50 mins
o Stop data at 7.0 mins.
o Rinse with DI water for 1 min at time 10 mins. ( or any time after sample is done
running)
After Running the sample



Save and run the method (see manual for detailed instructions)
Save and print electropherogram if printer works
Exit program and turn off instrument