SBI 4U
Enzyme Lab
Name: _________________________
Factors that Affect the Activity of Catalase
The enzyme catalase found in liver and potatoes catalyzes the decomposition of hydrogen peroxide (H2O2) to water and
oxygen gas.
Balanced Chemical Equation:
The factors that affect enzyme activity are __________________, _______________________, ____________________
and _____________________.
Controls for all Procedures
 5 variations for each factor
 2 trials (3 preferred) for each variation
 total enzyme volume = 3 mL (this can be all enzyme or a mix of enzyme and water)
 total substrate volume = 5mL (this can be all substrate or a mix of substrate and water)
 time the reaction for 1.0 min for each trial
 use the same equipment for each trial
How Enzyme Activity will be Measured
Procedure 1: Temperature 20°C and above
Procedure 2: Temperature 20°C and below
Procedure 3: Enzyme Concentration
Variation
Volume of
enzyme (mL)
Volume of water
[enzyme]
1
3
2
2.5
0
100%
0.50
2.5/3=83.3%
3
4
5
3
4
5
Procedure 4: Substrate Concentration
Variation
Volume of substrate
(mL)
Volume of water
relative [substrate]
actual [substrate]
1
5
0
100%
3 or 6%
2
SBI 4U
Enzyme Lab
Procedure 5: pH 7 and below (acidic)
pH
[H3O+] needed
[acid used]
initial [H3O+]
total volume
volume acid needed
# of drops
6
Name: _________________________
5
4
3
2
9
10
11
12
Procedure 6: pH 7 and above (basic)
pH
pOH
[OH-] needed
[base used]
initial [OH-]
total volume
volume base needed
# of drops
8