Chpt. 5 The Structure and Function of Macromolecules macromolecule ex. D NA ex. PROTEIN macromolecule Macromolecules (large molecules): Macromolecules (large molecules): Carbohydrates Lipids Proteins Nucleic Acids These are all: Polymers (many parts) constructed of Monomers (single part) Connecting Monomers- Hydrolysis Breaking apart polymer Adding water molecule Connecting MonomersDehydration reaction Hydrolysis Breaking apart polymer Adding water molecule Connecting MonomersDehydration reaction joining monomers w/ covalent bond loss of water molecule Hydrolysis Breaking apart polymer Adding water molecule Connecting MonomersDehydration reaction joining monomers w/ covalent bond loss of water molecule Hydrolysis Breaking apart polymer Adding water molecule Connecting MonomersDehydration reaction this requires cell to use energy (reaction occurs b/c of enzymes) Hydrolysis Breaking apart polymer Adding water molecule HydrolysisBreaking apart polymers Breaking apart polymer Adding water molecule HydrolysisBreaking apart polymers Breaking apart polymer Hydrolysis(break w/ water) Adding water molecule Disassemble & add a water molecule HydrolysisBreaking apart polymers Breaking apart polymer Hydrolysis(break w/ water) Adding water molecule Add a water molecule HydrolysisBreaking apart polymers Breaking apart polymer Hydrolysis(break w/ water) Adding water molecule Add a water molecule HydrolysisBreaking apart polymers Breaking apart polymer Hydrolysis(break w/ water) Adding water molecule Adding water molecule HydrolysisBreaking apart polymers Breaking apart polymer Hydrolysis(break w/ water) Adding water molecule Adding water molecule Macromolecules (large molecules): Carbohydrates Lipids Proteins Nucleic Acids Carbohydrates- CarbohydratesFunction = fuel Structure = 3 types: Types: 1) Monosaccharides Single Sugar molecule Which functional groups? isomers Types: 1) Monosaccharides a hydroxyl group is bonded to each carbon, except one; THAT carbon is double bonded to an oxygen atom = carbonyl Which functional groups? Monosaccharides - ALDOSE - C=O-H from end KETOSE- C=O off middle Monosaccharides function = fuel for cell use in most organisms ex. glucose Monosaccharides carbs. always multiple of CH2O ex. C6 H12 O6 FYI: Tend to form rings in aqueous solutions Monosaccharides Single Sugar Fuel for cellular function BTW: form rings in aqueous solution “carbon one” is always on end near the carbonyl Types: 2) Disaccharides 2)Disaccharides Structure = two sugar monomers (joined by a glycosidic linkage oxygen cov.bond formed between 2 carbons (monosacchs.) via. dehydrat.rx. 2) Disaccharides Types: 3) Polysaccharides structure = many monomers Polysaccharides Functions: •A) energy storage starch (plant) glycogen (animal) Polysaccharides Functions: B) Structural/ support cellulose chitin (chitin) Ncontaining “appendage” WHY are starch and cellulose different??? Do you see the difference? DIFFERENT STRUCTURE… DIFFERENT FUNCTION DIFFERENT STRUCTURE… DIFFERENT FUNCTION Macromolecules (large molecules): Carbohydrates Lipids Proteins Nucleic Acids LipidsTypes: Triglyceride (Fats) Phospholipid Steriods Why are these molecules hydrophobic ??????????????????? Lipids- (hydrophobic) 1) Triglycerie (fats) - functions: -energy storage -insulation / cushion Triglyceride Fats- structure 3 Glycerol (alcohol) + Fatty acid chain = hydrophobic •Energy storage •Cushion Fats- structure 3 Glycerol (alcohol) + fatty acid chains Fatty acid chain = hydrophobic •Energy storage •Cushion Cis arrangement unsaturated saturated fat Types of Lipids: 2) Phospholipids1 Glycerol (alcohol) + Types of Lipids: 2) Phospholipids- 1 Glycerol (alcohol) +2 fatty acid chains + Types of Lipids: Phospholipids- 1 Glycerol (alcohol) +2 fatty acid chains + phosphate group Types of Lipids: Phospholipids- 1 Glycerol (alcohol) +2 fatty acid chains + phosphate group Types of Lipids: Phospholipids•Phosphate group = hydrophilic Fatty acid chain = hydrophobic Cell membrane Hydrophilic head WATER WATER Hydrophobic tails Types of Lipids: 3) Steroids C-skeleton = 4 fused rings + functional group cholesterol, sex hormones, sheath of neurons Macromolecules (large molecules): Carbohydrates Lipids Proteins Nucleic Acids Amino Acids H amino group C carboxyl R 20 types ProteinsStructure = one or more polypeptides- polymer of amino acids Proteinsone or more polypeptidespolymer of amino acids Amino Acids H amino group C carboxyl Determined by various side R 20 types chains Amino Acids Evolutionary Significance All living things use various combinations of the SAME twenty amino acids. A VERY POWERFUL bit of evidence for the connection of all living things! Amino Acids H amino group C Determined by various side R chains carboxyl 20 types Proteinsfunctions = support, storage, signaling, transport of substances, enzymes. Proteins- 4 levels of structure 1) Primary- precise, linear sequence of amino acids. amino -O-O-O-O-O- carboxyl group group Sequence of a.a. determines HOW the protein works Form follows function Nonpolar Glycine (Gly) Methionine (Met) Alanine (Ala) Valine (Val) Phenylalanine (Phe) Leucine (Leu) Tryptophan (Trp) Isoleucine (Ile) Proline (Pro) Polar Serine (Ser) Threonine (Thr) Cysteine (Cys) Tyrosine (Tyr) Asparagine (Asn) Glutamine (Gln) Acidic Basic Electrically charged Aspartic acid (Asp) Glutamic acid (Glu) Lysine (Lys) Arginine (Arg) Histidine (His) Proteins: 4 levels of structure 2) Secondary- folded portions of chain/H-bonds -Alpha helix (coil) -Beta helix (pleated sheet) Sturdy Bounces back Abdominal glands of the spider secrete silk fibers that form the web. The radiating strands, made of dry silk fibers, maintain the shape of the web. The spiral strands (capture strands) are elastic, stretching in response to wind, rain, and the touch of insects. Spider silk: a structural protein Containing b pleated sheets Proteins- 4 levels of structure 3) Tertiary- -3-D -bonding between side chains (3 types) Proteins- 4 levels of structure 4) Quaternary-several polypeptide chains insulin Catalase - we will use this in our lab! Red bloodRed Normal cells are blood cell shape full of individual cell shape hemoglobin molecules, each carrying oxygen. Normal cells are full of individual hemoglobin molecules, each carrying oxygen. Red blood Red blood Fibers of abnormal cell shape cell shape hemoglobin deform cell into sickle shape. Fibers of abnormal hemoglobin deform cell into sickle shape. POINT! Sickle-cell hemoglobin Normal hemoglobin Primary structure Primary structure Secondary and tertiary structures Secondary and tertiary structures b subunit Quaternary Normal hemoglobin structure (top view) Function Molecules do not associate with one another; each carries oxygen. Quaternary structure Function Exposed hydrophobic region Sickle-cell hemoglobin Molecules interact with one another to crystallize into a fiber; capacity to carry oxygen is greatly reduced. b subunit Nucleic AcidsDNA RNA Programs ALL cellular function Nucleic AcidsComposed of: nitrogenous base, 5-Carbon sugar, phosphate group = NUCLEOTIDE