F.R.E.T. Experiment

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F.R.E.T. Experiment
Max Leslie
Why is this helpful?
• Reduces the
amount of
RADIOACTIVE
material needed
for lab
experiments!
What is FRET?
• Florescence Resonance Energy Transfer
• English please?
– FRET is a way to understand how close two molecules
are together
– This process includes one molecule that ABSORBS
florescence and one molecule that RELEASES
florescence
– The basis of this experiment is when the two
molecules moves closer together the light is
decreased and when the two molecules move away
from each other the amount of light is increased
LIGHT!
Molecule 1
Molecule 2
Molecule 1
Molecule 2
NO LIGHT
96 Separate Reactions!
• You can have 96 separate reactions occurring
at one time on one plate (3inches x 5inches)
Why not a gel?
• Gel option – far
fewer reactions
and it takes a lot
more time
• Quantification is
some what
subjective
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