Guidelines for Morphological Unknown

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Microbiology 20 Morphological Unknown Guidelines

You should be prepared to turn in your morphological unknown characterization by the end of lab on the due date. Check the syllabus for due date.

Out of a possible 30 points you will be graded as follows:

Successfully performing the following tests to characterize your unknown (10 pts):

Gram stain (Ex. 3-6)

Morphology & arrangement

Capsule stain (Ex. 3-8)

Endospore stain (Ex. 3-9)

Acid fast stain (Ex. 3-7)

Motility (via wet mount or hanging drop, Ex. 3-10)

Colony characteristics on nutrient agar plate (Ex. 2-2, pg. 56) o Color (observe color of colonies as well as any changes in color of agar) o Overall appearance o Margin o Elevation

Slant characteristics (Ex. 2-3) o Filiform, spreading, friable, or transluscent/transparent o Color

Broth characteristics (Ex. 2-4) o Pellicle o Turbidity o Sediment o Flocculence o Relative growth at 22 versus 37 o C

Adequately recording the results of each test (10 pts):

Your results should be recorded in color and/or sufficiently described and labeled

Indicate the total magnification for all microscopic images

It should be clear on what date each test was done and/or analyzed

Your results should be neat, well-organized, and easy to read

Correctly interpreting your results and describing your unknown (10 pts):

Correctly indicating what each result means (e.g., “pink” Gram stain = Gram-negative)

Brief summary or narrative of your results

Can include charts, tables or lists

If possible, choose identity of unknown or possible unknown(s) by comparing results with chart provided

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