You should be prepared to turn in your morphological unknown characterization by the end of lab on the due date. Check the syllabus for due date.
Out of a possible 30 points you will be graded as follows:
Successfully performing the following tests to characterize your unknown (10 pts):
Gram stain (Ex. 3-6)
Morphology & arrangement
Capsule stain (Ex. 3-8)
Endospore stain (Ex. 3-9)
Acid fast stain (Ex. 3-7)
Motility (via wet mount or hanging drop, Ex. 3-10)
Colony characteristics on nutrient agar plate (Ex. 2-2, pg. 56) o Color (observe color of colonies as well as any changes in color of agar) o Overall appearance o Margin o Elevation
Slant characteristics (Ex. 2-3) o Filiform, spreading, friable, or transluscent/transparent o Color
Broth characteristics (Ex. 2-4) o Pellicle o Turbidity o Sediment o Flocculence o Relative growth at 22 versus 37 o C
Adequately recording the results of each test (10 pts):
Your results should be recorded in color and/or sufficiently described and labeled
Indicate the total magnification for all microscopic images
It should be clear on what date each test was done and/or analyzed
Your results should be neat, well-organized, and easy to read
Correctly interpreting your results and describing your unknown (10 pts):
Correctly indicating what each result means (e.g., “pink” Gram stain = Gram-negative)
Brief summary or narrative of your results
Can include charts, tables or lists
If possible, choose identity of unknown or possible unknown(s) by comparing results with chart provided