LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIO MEDICAL INSTRUMENTATION SECOND SEMESTER – APRIL 2006 DB 6 BI 2951 - MICROBIAL TECHNOLOGY Date & Time : 26-04-2006/9.00-12.00 Dept. No. Max. : 100 Marks SECTION A Answer all the questions: - (102=20) 1. Define the following terms: - Chemoautotrophs, chemoheterotrophs, Photoautotrophs and Photoheterotrophs 2. Mention the three steps in immune response against invading microorganisms? 3. Comment on immunoglobulins. 4. Name some industrially useful microbes and their products. 5. Explain the method for the determination of vitamin B12. 6. What is a pure culture? What are the basic steps involved in obtaining a pure culture? 7. What are a) simple stain c) Gram’s stain b) Differential stain d) Acid fast stain 8. List out the contributions of Pasteur. 9. What are the characteristics of cytokines? 10. What is meant by opsonisation? SECTION B (410=40) Answer any four: 11. Write a note on some industrial products produced by fermentation technology. 12. Give an account of immunoelectrophoresis. Write the basic mechanisms of antigen-antibody reaction. 13. Write about the medical importance of Plasmodium species. 14. Write a note on microbial enzymes of industrial importance. 15. Bacteria are extremely diverse. Why? and How? 16. What are the characteristics of fungal diseases? SECTION C Answer any two: (2x20=40) 17. Write an account on immune response. 18. Give an account of types of reactors used in fermentation technology. 19. Explain how microbes are used in industrial production of vaccines and vitamins. 20. Write a note on culture media. Explain any two methods of bacterial culture used in laboratories. ____________