Microbiology lab homework #4
1- Immersion oil should be used when using the 100 x objective to a) improve resolution b) prevent diffraction of light c) all of the above d) none of the above
2- True or False
In experiment # 14 the capsule of Klebsiella pneumoniae can be observed by combining the methods of simple staining and the negative stain.
3- The exosporium or endospore coat is composed of a) Proteins b) Peptidoglycan c) Lipids d) None of the above
4- Which of the following is an acid fast microorganism? a) E. coli b) S. aureus c) S. pneumonia d) Mycobacterium smegmatis
5- True or False
Endospores are produced by the genus Bacillus and Clostridium.
6- All the following are gram negative bacteria except a) Proteus vulgaris b) Staphylococcus aureus c) Neisseria gonorrhoeae d) Salmonella typhi
7-To destroy all spores by heating, Microbiology experts recommend a temperature
of 121
0
C and 15 psi of pressure for 15 to 20 minutes. Which type of sterilizing
technique best accomplishes this task? a) Autoclaving b) using a Dry oven c) Filtering by vacuum d) Boiling

8- Methylene blue is the a) primary stain for the Gram stain b) primary stain for the endospore stain c) counter stain for the Gram stain d) counter stain for the acid fast stain
9- Why is staining of the capsule difficult at times and how is it accomplished?
10- What is a capsule and what are 2 functions of it?
11- What are 2 methods used to stain spores and how do they differ?