MICROBIOLOGY TEST #1
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This is just a guideline to help you study. Information from lab manual, handouts (look at my
website www.jcapers-irsc.weebly.com , lecture or discussion in class, as well as lab resources
online will be covered.
The exam will consist of two parts: short answer (52 points) and lab practical (48 points),
total of 100 points
Short answer essay
Week 1:
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Know the parts and function of microscope
Define (and/or) calculate total magnification, resolving power, parfocal, numerical aperture
Know how to use and care of microscope
Know basic shapes of bacteria
Week 2:
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Know the different methods to determine motility
Understand true motility versus brownian movement, and water movement
How does dark field microscopy work?
Know how to change from bright field to phase contrast and dark field
Distinguish and compare advantage/disadvantage between bright field, phase contrast, and
dark field microscope
Can a bacteria have more than one flagella?
Of the 2 bacteria we tested, which one has true motility? What were the results of the
agar method?
Week 3:
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Know the different ways to obtain a pure culture (spread plate, streak plate, pour plate)
How do you make a streak plate?
Know how to make a smear on a slide from specimen growing on agar (solid) and a broth
(liquid)
Understand why stains work. Do basic stains work better? Why?
Know different between simple stain and differential stain
Week 4:
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Know the procedures for doing the gram stain and acid fast stain, know what happens at
each step (don’t worry about times)
Understand the concept of how both the gram stain and acid fast stain work
Know the differences in morphology of cell wall of gram positive and gram negative bacteria
Know the differences in morphology of cell wall of acid fast positive and acid fast negative
bacteria