MICROBIOLOGY 20 BIOCHEMICAL UNKNOWN IDENTIFICATION REPORT
Name: ___________________________
Date: ______________
Unknown Number: __________________
STAINING
PROCEDURE
BROTH
SLANT
SKETCH OR PICTURE
Magnification
Gram Stain
Gram Stain (____X)
Color
Interpretation (+ or -)
Morphology
Arrangement
Acid Fast Stain
Color
Interpretation (+ or -)
Acid Fast Stain (____X)
Capsule Stain
Capsule Stain (____X)
Date:_____________
Interpretation (+ or -)
Endospore stain
Endospore stain (___X)
Date:_____________
Interpretation (+ or -)
Comments:
COLONY CHARACTERISTICS
Diameter (mm)
Pigment Color
Margin
Entire Colony Appearance
Relative Growth (More, less, equal)
25 OCELSIUS
BROTH GROWTH: CHECK ALL THAT APPLY
Turbidity
Pellicle
Sediment
37 OCELSIUS
Flocculance
Pigment
SLANT GROWTH: Describe growth pattern and color on a slant and draw a sketch.
Microbiology 20 Biochemical Unknown Report
1
Determination of Motility: This unknown is motile (circle answer):
Motility was determined by (circle all that apply):
Hanging drop
YES
Wet mount
NO
Motility agar
Comments____________________________________________________________________________
BIOCHEMICAL TESTS
Test
OF-Glucose
Date
Results/Observations
Interpretation
Unsealed Tube:
Sealed Tube:
Phenol Red
Glucose
Phenol Red
Sucrose
Phenol Red
Lactose
Methyl Red
VogesProskauer
Catalase
Oxidase
Nitrate
Reduction
Gas:
YES or NO
Color after reagents A & B?
If Zinc added, color change?
Citrate
Decarboxylation
of:
Phenylalanine
Deamination
Starch
Hydrolysis
Urea Hydrolysis
Gelatin
Hydrolysis
SIM Medium
Sulfur reduction
SIM Medium
Indole
2
Microbiology 20 Biochemical Unknown Report
+/- or
Symbol
Other Tests
Date
Results/Observations
Interpretation
+/- or
Symbol
Flowchart: Construct a flowchart below with all possible GRAM POSITIVE OR GRAM NEGATIVE
unknowns from the tables provided and indicate which unknown you have IDENTIFIED.
FLOWCHART
Microbiology 20 Biochemical Unknown Report
3
Summary of the Identification Process
Conclusion
Unknown number ______ is the following organism:_________________________________________
Additional Comments
4
Microbiology 20 Biochemical Unknown Report