IDENTIFYING IMMUNE CELLS Preparing a Blood Smear: 1. Place one drop of peripheral blood on one end of a clean slide . 2. Position a clean slide at 45 degree angle in front of the drop until the edge of this Slide touches the blood . 3. Move the angled slide across the bottom slide pulling the blood along. 4. Air dry for ~ 10 minutes Staining White Cells 1. 2. 3. 4. 5. Dip the blood smear slide into absolute alcohol. Remove the dipped slide, wick the excess liquid, air dry. Place the fixed slide into Giemsa Stain for 3-5 minutes (note time carefully). Remove from stain, wick, place into dd water for time equal to time spent in stain. Remove, wick, air dry. Identifying Immune Cell Using 40x and/or oil immersion identify the following cells: Quantifying Immune Cells Choose three equally spaced areas on the slide (instructor will demonstrate) and count the number of 1. all the cells seen in the total of all areas 2. keep track of the # of RBC, and each of the white cell types 3. calculate the % present of each of the white cells.