Announcements
• Lab quiz this week on PCR & DNA.
• Get started on 30 Days experiment,
create schedule/timeline/calendar,
use preliminary data DRAFT2 Figures
• Craft email message to seek mutant
DNA from scientist (positive control)
Sent from msu.edu address, only one author, short
Dear Dr. Rubenstein,
I just read your 2006 PNAS paper “Regulatory interactions of N1303KCFTR”, and the results of your experiments were very helpful to us.
Currently, here at Michigan State, we are developing a PCR method to
detect various CFTR mutations including the presence of N1303K and we
were hoping that you might be able to share with us cells or a DNA sample
containing this mutation. We would be very grateful if you were able to
assist us.
Please let us know if it is possible to get some DNA containing the N1303K
mutation from you.
Thanks,
John Stein,
Michigan State University, Lyman Briggs College of Science
DNA Structure
LB 145
February 1, 2011
Ribose Sugar
Nitrogenous Bases
Phosphate
DEOXYRIBONUCLEIC ACID
DNA is called DE-oxy…Why?
• A. Each nucleotide incorporates 10 oxygen
atoms
• B. Nucleotides function anaerobically (don’t
require oxygen to function)
• C. Oxygen donates electrons to the
nucleotide during DNA replication reactions
• D. A hydroxide group is missing from the
ribose sugar in each nucleotide
Nucleotide Polymer (5’ & 3’)
Phosphodiester bond is an
example of what kind of
reaction?
•
•
•
•
A. Condensation Reaction
B. Elimination Reaction
C. Substitution Reaction
D. Diels-Alder Reaction