Lab Report Instructions ARISE GM Foods The Lab write-up is the culmination of your experiments and is therefore very important. Even if you have not finished collecting the data, you can start with the write-up and save some time later when you finish getting data and analyzing it. Everyone will be required to write up their lab separately, you may work with group members or others in the class, but this must be your individual writing, no copying!!! This lab report does not have to be typed, but it is a good idea and makes changing something much easier. Notice that the write-up is in paragraph form. NOT QUESTION AND ANSWER FORMAT. It should be written so that the reader can duplicate the activity and results if they so desire So lets begin! 1. You should always start with a HEADING INFO. The following items are required on your title page: • Your Name and Period • Title of lab • Who you worked with • Teacher’s Name 2. For the first part of your write up, there is the INTRODUCTION. This is where most students tend to lose points, so be careful! For this lab, your introduction will consist of background information, observations or questions and your hypothesis for what you expect. In a few words tell what you already know or have found about the problem that will let you make an educated guess. This is your background information from the text, teacher, or other sources. It gives the reader an understanding of underlying principles and content information of the laboratory. http://www.donnayoung.org/apologia/ecw/lab/labhow-cr.htm • Introduce the lab by stating the purpose of this lab • Explain what Genetically Modified foods are • Include your hypothesis at the end to explain what you expect (The hypothesis can be stated as an "If..., then..." statement. The 'If' part of the statement is based on related facts that you know to be true. The 'then' part of the statement is an educated guess on the outcome of the experiment. The hypothesis does not have to guess the correct outcome, but the experiment must be set up to test the hypothesis.) Here is a sample hypothesis for this lab: The purpose of this lab was to determine if vegetables found in our cafeteria have the transgene that makes them resistant to the herbicide Round-up. 3. Next is the MATERIALS AND METHODS, everything should be done in paragraph form, as with the introduction. • Give a detailed, but brief account of how you extracted DNA from the foods. Include quantities of solutions and temperatures for each step • Explain what primers you used for the PCR and what cycles were run on the thermocycler • Explain what controls and replications exist 4. Don’t forget about the RESULTS section. • Include a nice picture of your electrophoresis gel, labeled as to what samples are in which lanes. Include observations: measurable information that comes to you through your senses • Explain what your controls showed and each of your samples (ie. Did the positive control show the correct size PCR band?) 5. And last, but the most important: your analysis of the data and CONCLUSIONS. • Explain what you found about the foods • State whether this supports your hypothesis and explain how. • Indicate where problems or sources of error occurred or things that were not expected and give suggestions for changes in your next experiment. • Come up with some new questions or hypotheses that you thought of during or as a result of this experiment. You will be graded on this assignment with the following rubric so keep these in mind. After you have written the lab report, read through it and give yourself a grade for each of the sections, marking this on the rubric and turn this in with your lab report. Name: Date: (1) what were you thinking? (5) excellent work (4) good work (3) almost there (2) not quite Introduction and Hypothesis Association between the problem and the predicted results is direct and relevant and supported by previous learning. All variables are clearly explained. Hypothesis clearly refutes or defends established knowledge and is fully supported. Student demonstrates facility in the use of scientific concepts and vocabulary. Grammar, spelling, and presentation are faultless. Association between problem and predicted results. Made attempt to explain key variables Hypothesis has some relationship to established knowledge but is not supported. Scientific concepts and vocabulary used, but contains errors. Noticeable errors in grammar, spelling or scientific vocabulary usage. Unreasonable association between problem and predicted results. Hypothesis weakly addresses the problem and is not based on previous learning. Results are not supported. Defends or challenges established knowledge Scant use of scientific concepts and vocabulary. Noticeable errors in grammar, spelling. Methods and Results All significant data measured, Appropriate controls explained. Data recorded effectively and efficiently. Data table is welldesigned, easy to read and appropriate. Reasonable association between the problem and the predicted results. Key variables are operationalized Hypothesis has a reasonable relationship with established knowledge; this relationship is generally supported. Scientific concepts and vocabulary used without significant error. Grammar, spelling and presentation has few if any flaws. All significant data measured, Appropriate controls included. Data recorded effectively and efficiently. Data table welldesigned and appropriate to the task requirements. All significant data measured. Appropriate controls missing. Data recorded effectively The data table is relevant to the task requirements Most data are collected, lacks controls. Data are recorded in a manner that threatens reliability Data table incomplete or contain inconsistencies Data are inaccurate Data are haphazardly recorded Data table missing Analysis and Conclusions Analysis is complete and accurate. Conclusion precisely stated, relates directly to support or refute the hypothesis. Conclusion relates the study to general interest, other studies that have been or could be conducted. Suggests further studies based on findings. Conclusion precise, related to the hypothesis Conclusion attempts to translate the conclusion to make it relevant to the original problem The conclusion related to general interest and other studies Analysis lacks cohesion. Conclusion too general or overreaches the data analysis Conclusion uses the language of the experiment but does not translate. conclusion to its relevance to the original problem. Inconclusive, or conclusion not warranted by data analysis. Impossible to understand if hypothesis supported or refuted. Analysis is complete and accurate. Conclusion precisely stated, relates directly to support or refute the hypothesis. Conclusion relates the study to general interest, other studies that have been or could be conducted Lacks hypothesis. Proposed resolution to the problem is not supported by previous learning. Excessive errors in grammar, scientific language usage, or spelling. Teacher evaluation Student evaluation Total