Acrylamide mix ( Acrylamide and bisacrylamide)
TEMED(N,N,N’,N;-tetramethylethylnediamine)
Sodium Dodecyl sulfate (SDS)
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.Robert Insall
Assessment Number*
2032
Date of Assessment
17/2/02
Signature
Assessor
Soha Zouwail
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK
ACTIVITY
2
PERSONS WHO MAY BE AT
RISK
List names where possible
3
ACTIVITY
ASSESSED
8th Floor labs
Frank Zimmerman,Jason King , Margarita Tchibalina,Robert Insall ,Soha
Zouwail, and may be other members of both Robert’s &Laura’s labs
Use of SDS -Page gel for protein.
Attach copies of data sheet(s)
MATERIALS
INVOLVED
NAME
AMOUNT**
HAZARD
RISK PHRASES
Acrylamide mix
( Acrylamide and
bisacrylamide)
4 ml of 30%
Acrylamide
mix
Toxic&
Carcinogenic
TEMED(N,N,N’,N;tetramethylethyl
nediamine)
4 ul
Irritant
Sodium Dodecyl
sulfate (SDS)
0.1 ml of
10%
solution
Irritant
Harmful if
swallowed
R39/27Very
toxic:danger of
irreversible effects
in contact with skin
R23/24/25 Toxic by
inhalation ,in contact
with the skin &if
swallowed
R45 may cause
cancer
R46 may cause
heritable genetic
damage
R 46 Danger of
serious damage to
health by prolonged
exposure
R11 highly
flammable
R20/22 harmful by
inhalation or if
swallowed
R34 causes burns
R 36/37/38 Irritant
to eyes ,respiratory
system &skin
R41 risk of serious
damage to eyes
R42 may cause
sensitisation by
inhalation.
R20/22 harmful by
inhalation &if
swallowed
R36/37/38 irritation
to eyes, respiratory
system, &skin.
HAZDAT
NO***
BIOSCIENCESNO***
Ammonium
persulphate (APS)
0.1 ml of
10% APS
Hydroxymethylamino
methane(Tris)
2.5 ml of 1.5 Irritant
M Tris
5
R8 contact with
combustible
material may cause
fire.
R22 harmful if
swallowed.
R34 causes burns.
R42/43 may cause
sensitisation by
inhalation or skin
contact.
R36/37/38 Irritant to
eyes, respiratory
system, &skin.
INTENDED
USE
Give brief details and attach protocol/instructions
6
Irritant
Harmful if
swallowed
Preparation & use of gels containing different
percentages of acrylamide to separate proteins of
different sizes.
RISKS to HEALTH and SAFETY from
INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
There is risk of inhalation of powder when preparing SDS solution. There is risk of skin
contamination when using acrylamide mix, or skin burn when using the TEMED & APS
solutions.
7
CONCLUSIONS ABOUT
RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The level of risk is not acceptable and control measures should be followed.
8
CONTROL
MEASURES
Additional to Good Chemical Practice
The sodium dodecyl sulphate (SDS) should be weighed in the fume hood on the 7 th floor whilst
wearing mask, gloves and lab coat.
The acrylamide mix &TEMED should be all used while wearing gloves.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Personnel involved in the clean up procedure must be adequately trained and experienced in
handling carcinogenic materials.
10
MONITORING
Performance of control measures,
Check fume hood is working properly before use.
Personal exposure
Health Surveillance
Wearing lab coat and gloves during the whole
procedure.
11
WASTE DISPOSAL
PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste
Disposal.
Polymerised Acrylamide can be placed in yellow bags for incineration. Any of the other
chemicals could be disposed in the drain followed by large amount of water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
February 2007
13
EMERGENCY
ACTION
To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
TO CONTROL
HAZARDS
Any spillage of acrylamide should be carefully cleaned whilst wearing gloves and protective
clothes and the waste should be discarded as special waste (contact Brian Facer in stores).
Treat contaminated surface with household bleach and then wash thoroughly with water. If
APS or TEMED has been spilled, sources of ignition and combustible material should be
eliminated & absorbant material should be spread on the spill.
Evacuation, protection for personnel involved in clean-up, Special First Aid
TO PROTECT
PERSONNEL
If any of the hazardous chemicals comes in contact with the skin, remove people from source
of contamination, bring injured person under a shower, wash contaminated part of the body for
a minimum of 10 minutes, remove any contaminated clothing and seek medical advice. Ensure
that the person involved in the clean up is wearing gloves and lab coat. Breathing apparatus
may also be required.
TO RENDER SITE OF EMERGENCY Clean-up/decontamination
SAFE
For air contamination: evacuate the place and ventilate room as much as possible to dispel
vapour.
For flammable materials: shut off all sources of ignition in the room, evacuate all people
immediately, contact security or ring fire alarm.
CONTACT
R. Insall
PHONE
42507
10.10.00
*
Prefix T is used for Teaching Assessment Number.
**
List the amount by weight of the substance used. (for liquids eg; 100 mls 1M
Sodium Hydroxide =
4g).
***
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT
SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY
OFFICE.