Acrylamide/bisAcrylamide
Tris
Glycine
AmmoniumPersulfate
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Dr.J.E.Turnbull
Assessor
Laine Wallace
Assessment Number*
JETLW4 (#2048)
Date of Assessment
19/06/02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
List names where possible
5th Floor Biosciences Tower
Laine Wallace, Dr.J.E.Turnbull,K.Drummond,Z.Schofield,Dr.E.Yates, Dr,S Guimond,
Dr.Z.Huang, Dr.A.Powell, J.Henstock, Dr. T. Kinnunen
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Glyco PAGE separation of saccharides.
AMOUNT
max/stored
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
Acrylamide/bisAcrylamide
0.5-10ml
Toxic
Sensitiser
R45 : May cause cancer
R46 : May cause heritable
genetic damage
R60 : May impair fertility
R63: possible risk of harm to
the unborn child
R24/25 : Toxic in contact
with skin and if swallowed
R36/38 : Irritating to eyes and
skin
R42/43: May cause
sensitisation by inhalation an
din skin contact
R48/23/24/25 : Toxic :
danger of serious damage to
health by prolonged exposure
in
Tris
200g
Irritant
R36/37/38: Irritating to eyes,
respiratory system and skin
Glycine
40g
N/A
N/A
HAZDAT NO***
BIOSCIENCESNO***
AmmoniumPersulfate
100mg
Harmful
|Sensitiser
R8 : Contact with
combustible material may
cause fire
R22 : Harmful if swallowed
R42/43: May cause
sensitisation by inhalation an
din skin contact
R36/37/38: Irritating to eyes,
respiratory system and skin
TEMED
20ul
Corrosive/
Harmful/
Flammable
R11 : Highly Flammable
R20/22: Harmful by
inhalation and if swallowed
R34 : Causes burns
AceticAcid
70ml
Corrosive/
Flammable
R10 : Flammable
R35 : Causes severe burns
EthyleneGlycol
35ml
Harmful
R22 : Harmful if swallowed
Bromophenol Blue
<10mg
Harmful
Phenol red
<100ul
Irritant
R21: Harmful in contact with
skin
R36/37/38: Irritating to eyes,
respiratory system and skin
R36/37/38: Irritating to eyes,
respiratory system and skin
5
INTENDED USE**
Give brief details and attach protocol/instructions
Acrylamide/bis-Acrylanmide – gel constituant to separate saccarides
Tris – buffer
Glycine – gel and buffer constituant
Ammonium persulfate – gel polymerization Activator
Temed – gel polymerisation catalyst.
Acetic Acid – to adjust pH
Ethylene Glycol – Constituant for running buffer
Bromophenol blue – colour marker to determine location of saccahrides
Phenol red – colour marker to determine location of saccharides
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Acrylamide/bis-Acrylanmide – Risk of harm due to splashes of liquid reagent to unprotected eyes and skin. Small
risk of inhalation. User may become sensitised with use.
Ammonium persulfate – Small risk of harm by inhalation. Risk of harm due to splashes to the unprotected skin
and eyes. User may become sensitised with use.
Temed – Corrosive – causes burns. Small risk of harm from inhalation. Risk of harm from splashes to unprotected
skin and eyes Flammable – vapour may trail towards sources of ignition.
Ethylene Glycol – Small risk of inhalation. Risk of harm from splashes to skin and eyes.
Acetic Acid – Corrosive risk by inhalation. Risk of burns due to splashes to unprotected skin and eyes.
Flammable – refrain from use by ignition sources.
Phenol red – Small risk of irritation by inhalation. Risk of harm due to splashes to unprotected skin and eyes.
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Due to the nature of some of the chemicals listed in this assessment there is a small risk . But due to the small
amounts used and as long as measures laid out in section 8 are followed then the level of risk is acceptable. Non
of the chemicals used can be replaced with other “ less hazardous” reagents.
8
CONTROL MEASURES
Additional to Good Chemical Practice
Laminar flow hood recommended for manipulation of acrylamide powders.
Wear labcoat, safety glasses and chemical resistant gloves.
Be aware of and avoid sources of ignition.
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
One to one initial instruction
10
MONITORING
Performance of control measures,
Users of Respiratory sensitisers need to be registered with the HSU and Occupational Health PRIOR to
commencement of work, to enable assessment, monitoring of exposure and possible subsequent sensitisation.
Instruction given as to importance and application of control measures. Training given to aid recognition of initial
symptoms of sensitisation.
Personal exposure
Health Surveillance
Exposure to sensitisers - Lung function tests and health
review as instructed by Occupational Health. Records
kept of outcome courtesy of Occupational Health.
Exposure to sensitisers - Lung function tests and
health review as instructed by Occupational Health.
Records kept of outcome courtesy of Occupational
Health.
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Unused acrylamide solutions should be polymerised with APS and TEMED prior to disposal by incineration.
Used polymerised acrylamide gels should be disposed of by incineration.
Other reagents, ammounts listed within the protocol can be disposed of down the sink with copious amounts of
water.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
19/06/07
13
EMERGENCY ACTION
TO CONTROL HAZARDS
To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Small amounts of reagents listed within this protocol can be mopped up with absorbant material ( tissue ) .
Large spills of Acrylamide should be treated/mopped up with an adsorbant material,
TO PROTECT PERSONNEL
Evacuation, protection for personnel involved in clean-up, Special First Aid
Personal contact with listed reagents should be treated immediately by flushing with copious amounts of water
before seeking medical advice.
Respiratory difficulty should be treated immediately by moving to area of well ventilated clean air and seeking
medical advice if deemed necessary due to unresolvement of symptoms.
Contaminated clothing should be immediately removed.
Clean-up/decontamination
TO RENDER SITE OF EMERGENCY SAFE
Areas of spillage should be cleaned with a diltue decon solution
Acrylamide : Large spills warrant evacuation. Use of self-contained breathing apparatus, rubber boots and heavy
duty chemical resistant rubber gloves. Spill should be mopped up with absorbant material, swept up into a bag,
held for specialist waste disposal by Departmental Stores.
Ventilate area and wash spill with diltue decon solution after material pick up is complete.
Small spllls od acrylamide should be mopped up immediately, placed in a bag for specialist waste disposal. Area
cleaned with diulute decon solution.
CONTACT Dr.J.E.Turnbull
PHONE
47527
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.