Name of supervisor
Assessor
Signature
Assessment Number* 2038
Date of Assessment 18.02.02
Signature
Notes A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment .
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form .
Use a continuation sheet to expand any section of this form in hard copy version.
1 LOCATION OF THE WORK ACTIVITY 8 th Floor labs
2 PERSONS WHO MAY BE AT RISK
List names where possible JA Woodings, CC Costa, LM Machesky, Friederike Bathe
3 ACTIVITY ASSESSED Preparation of actin from rabbit skeletal muscle acetone powder extended protocol : extraction of myosin and limited digestion to HMM
(Heavy Meromyosin)
Attach copies of data sheet(s) 4 MATERIALS INVOLVED
NAME AMOUNT**
Buffer G constituents listed below
1L
HAZARD RISK PHRASES HAZDAT NO*** BIOSCIENCESNO***
2mM Tris 2 ml Irritant R36/37/38 Irritating to eyes respiratory system and skin
0.5mM DTT 77mg Irritant
0.2mM CaCl
2
0,2ml Irritant
R36/37/38 Irritating to eyes respiratory system and skin
R22 Harmful if swallowed
R34 may causes burns
R36 Irritating to eyes

0.02%(w/v) NaN
50mM KCl
2mM MgCl
2
3
0,2g
100mL
100mL
Toxic
R28 very toxic if swallowed
R32Contact with acid liberates very toxic gas
R48/23/24/25 Danger or serious damage to health by prolonged exposure through inhalation ,in contact with skin and if swallowed
Non hazardous
Irritant R36/37/38 Irritating to eyes respiratory system and skin
Non hazardous
Ingestion and inhalation may be fatal
Material is readily absorbed through the skin
R4 forms very sensitive explosive metallic compounds
R20/21/22 harmful by inhalation, contact with skin and if swallowed
0.8M KCl
Extension: Myosin preparation
The buffers for the extraction of myosin and subsequent digest to
HMM contain no hazardous substances, which are not already covered by other
COSSH-forms, except: alpha- Chymotrypsin
100ml
10 mg Irritant
5 INTENDED USE
Give brief details and attach protocol/instructions
To prepare purified actin and skeletal myosin fragment (HMM)
R36/37/38 Irritating to eyes respiratory system and skin

6 RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
Risk of skin contamination or inhalation when making buffer G and chymotrypsin.
7 CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Level of risk not acceptable and can be prevented by using the following control measures.
8 CONTROL MEASURES
Additional to Good Chemical Practice
Wear chemical-resistant gloves and chemical safety goggles when making the buffer.
Use a well ventilated area or fume cupboard
9 INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
Training in handling toxic materials and use of the fume cupboard.
10 MONITORING
Performance of control measures,
Personal exposure Health Surveillance
11 WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
0,02% NaN
3
is a very dilute solution. It is acceptable to wash it down the sink with a lot of running water.
12 REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
5 years Feb 2007
13 EMERGENCY ACTION
TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
If the stock solution of NaN
3
is spilled, then mop up with absorbant material and place in yellow bag for incineration.

TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid
Wear breathing apparatus and protective clothing in case of fire
In case of skin contact,: wash with water and soap
In case of contact with eye: wash with copious amount of water seek medical advice
In case of swallowing: seek medical advice
In case of inhalation remove to fresh air seek medical advice
TO RENDER SITE OF EMERGENCY SAFE Clean-up/decontamination
Spilled material should be carefully moistened with water and wiped up with absorbant paper which should be placed in yellow bag for incineration. Ventilate the area
42504
10.10.00
* Prefix T is used for Teaching Assessment Number.
** List the amount by weight of the substance used. (for liquids eg; 100 mls 1M Sodium Hydroxide =
4g).
*** Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.