Acrylamide/bis-acrylamide
Sodium dodecyl sulphate(SDS)
Hazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of
supervisor
Professor GS Besra
Assessor
Dr Lynn G Dover
Assessment Number*
2021
Date of Assessment
16/4/02
Signature
Signature
Notes
A School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1
LOCATION OF THE WORK ACTIVITY
2
PERSONS WHO MAY BE AT RISK
E103
List names where possible
Dr Lynn G Dover
Dr William N Maughan
Dr Sudagar S Gurcha
Dr Petr Illarionov
Dr Mark Guy
Dr Henri Strating
Mr Derek Lonergan
Mr Alistair Brown
Mr Kevin Gibson
Mr Ian Daniels
Miss Rosalind Wilson
Miss Suzanne Senior
Miss Janet Redman
Mr Massoud Akkhtar
3
ACTIVITY ASSESSED
4
MATERIALS INVOLVED
NAME
Polyacrylamide gel electrophoresis of proteins & lipoglycans
AMOUNT
max/store
d
Attach copies of data sheet(s)
HAZARD
RISK PHRASES
Acrylamide/bis-acrylamide
500g
Carcinogenic
Toxic
R: 45-46-24/25-48/23/24/25
S: 53-45
Sodium dodecyl sulphate
(SDS)
1kg
Irritant
Harmful
Serious
damage to
eyes
R: 20/22-42-36/37/38-41
S: 26-36-22
methanol
2.5 l
Harmful
Toxic
Flammable
R: 11-23/24/25-39/23/24/25
S: 7-16-36/37-45
Glacial Acetic acid
2.5 l
Flammable
Causes burns
R: 10-35
S: 23-26-45
HAZDAT NO***
BIOSCIENCESNO***
Coomassie Brilliant Blue G
10g
Irritant
R: 36/37/38
S: 26-36-22
Bromophenol Blue
10g
Irritant
Harmful
R: 21-36/37/38
S: 26-36-22
Tetramethylethylenediamine
(TEMED)
100ml
Harmful
Causes burns
Flammable
R: 11-20/22-34
S: 16-26-36/37/39-45
Ammonium persulphate
100g
Harmful
Causes burns
May ignite
combustible
material
R: 8-34-22
S: 17-26-27-36/37/39
Dithiothreitol
100g
Irritant
Harmful
R: 36/37/38
S: 26-36
Glycerol
2.5 l
Irritant
R: 36/38
S: 26-36
Urea
1kg
Harmful
Irritant
R: 40-36/37/38
S: 26-36-22
5
INTENDED USE**
Give brief details and attach protocol/instructions
Polymerisation of polyacrylamide gels mostly containing SDS and sometimes up to 1.5M urea using 0.1%
TEMED and >0.1% ammonium persulphate
Samples to be prepared in buffer containing 10% glycerol, 100mM dithiothreitol and 0.1% bromophenol blue and
are often boiled using a PCR thermocycler.
Acetic acid (10%) and methanol (30%) to be used in fixing and de/staining. Stain contains 0.1% coomassie blue
6
RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding
mothers especially at risk?
Acetic acid: Flash point 40C
Methanol: Flash point 11C
TEMED: Flash point 10C
7
CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
Acceptable level of risk. Cannot substitute chemicals. See below for control measures
8
CONTROL MEASURES
Additional to Good Chemical Practice
Use Nitrile gloves and eye protection for handling acrylamide-containing solutions.
Wear face mask for dispensing SDS powder
Solutions of Acrylamide will be purchased in order to decrease the risk of inhalation
9
INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
New staff/students to be trained by postdoctoral fellows where applicable.
10
MONITORING
Performance of control measures,
Personal exposure
Health Surveillance
Personal exposure should not occur if the control
measures are observed.
As Personal exposure.
11
WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Polyacrylamide gels will be disposed of as solid waste
Waste acrylamide solutions will be polymerized and disposed of via solid waste
Destain will be disposed of via non-halogenated solvent waste route.
12
REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
Update names of users as changes occur
13
EMERGENCY ACTION
TO CONTROL HAZARDS
To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Use absorbent on spills of buffers, acrylamide and stain-destain solutions. Dispose absorbent from acrylamide
spills as Special Waste and thoroughly wash affected area. Shut off sources of ignition where glacial acetic acid
and methanol are involved.
TO PROTECT PERSONNEL
Evacuation, protection for personnel involved in clean-up, Special First Aid
Inform other laboratory members to avoid the affected area during cleaning
Those involved in cleaning should wear appropriate gloves (see above)
TO RENDER SITE OF EMERGENCY SAFE
See control hazards and personnel protection
CONTACT
Clean-up/decontamination
Simon Ward
GS Besra
Ext. 45420
PHONE 58125
10.10.00
*
**
***
Prefix T is used for Teaching Assessment Number.
Please include amount of chemicals used and how.
Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.