Lab Report
Lab Report
Enzyme Kinetics Lab
Answer the following questions about the results of this lab activity. Record your answers in the
boxes with the questions. It is important to discuss how changes in absorptance over time
explain the activity of an enzyme.
Send your completed lab report to your instructor. Don’t forget to save your lab report to your
computer!
The relative rate of enzyme activity can be calculated with the following formula:
Spectrophotometer absorptance 1 - Spectrophotometer absorptance 2 = Relative rate of activity
Send your completed lab report to your instructor. Don’t forget to save your lab report to your
computer!
Activity 1 – Effect of Substrate on Enzyme Activity
Answer the following questions about the results of this lab activity. Record your answers in the
boxes with the questions.
Sample
Absorptance
Spectrophotometer
Reading 1
Starch
Raffinose
Cellulose
Dextrin
Spectrophotometer
Reading 2
Difference
1. Explain how differences in the relative rate of activity can be used to
determine the specificity of an enzyme for a substrate.
2. Explain which carbohydrate is most specific for alpha-galactosidase based on
your data.
3.
Hypothesize why two or more molecules can be used as a substrate for one
enzyme.
Activity 2 - Distinguishing cell types and Activity
Answer the following questions about the results of this lab activity. Record your answers in the
boxes with the questions.
Sample
Absorptance
Spectrophotometer
Reading 1
Spectrophotometer
Reading 2
Difference
pH 1
pH 3
pH 5
pH 7
pH 9
1. Explain which pH is optimal for alpha-galactosidase activity with a starch
substrate.
2. Draw a graph of your data below using the Chart feature in the Insert tab
of Word. The graph should have relative activity on the Y-axis and pH on
the X-axis.
3. Hypothesize how a particular pH may cause an enzyme to best less
effective at carrying out chemical reactions.
Activity 3 - Effect of Temperature on Enzyme
Activity
Answer the following questions about the results of this lab activity. Record your answers in the
boxes with the questions.
Sample
Absorptance
Spectrophotometer
Reading 1
Spectrophotometer
Reading 2
Difference
30oC
40oC
50oC
60oC
70oC
1. Explain which temperature is optimal for alpha-galactosidase
activity with a starch substrate.
2. Draw a graph of your data below using the Chart feature in the Insert
tab of Word. The graph should have relative activity on the Y-axis
and temperature on the X-axis
3. Hypothesize how a particular temperature may cause an enzyme to
best less effective at carrying out chemical reactions. Hypothesize
what the graph would show if more data points were taken for
temperature at the optimum range.