MCB 730: MOLECULAR AND CELLULAR

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PBIO 3300/5300: PLANT GENETICS

(Class Numbers 10242 & 10243 respectively – 3.0 Credit Hours)-Fall 2015

Time & Place: Monday, Wednesday and Friday 8:35 – 9:30 AM; Grover E218

Instructor: Dr. Allan Showalter

Course web site: http://www.ohio.edu/plantbio/staff/showalte/PBIO 3300 & 5300/

Text: Introduction to Genetic Analysis (11th edition) ©2015 by Anthony J. F. Griffiths et al.

Prerequisites: BIOS 1700 or PBIO 1140

Course Description:

The purpose of this course is to provide students with an understanding of the fundamentals of plant genetics. Topics include Mendelian and non-Mendelian genetics, gene structure and function, genomics, plant breeding systems, population genetics, and evolution.

Learning Outcomes:

1. Understand the basic principles of Mendelian and non-Mendelian genetics and be able to use those principles to solve problems.

2. Understand and be able to explain the fundamentals of transcription and translation and the enzyme complexes involved.

3. Learn how genetic and molecular genetic research is done and be familiar with the techniques involved and what they do.

4. Understand how plant genetics differs from animal genetics.

Grades will be based on the following:

1. A midterm exam on the first third of the course (100 points).

2. A midterm exam on the second third of the course (100 points).

3. A comprehensive final exam (100 points).

4. Homework (100 points). See assigned homework problems and instructions for completing them on page 3 of the syllabus.

5. Graduate students enrolled in the course will additionally have to submit an 8-10 page double spaced paper (50 points) on a genetics topic approved by the instructor. The paper must follow the format of a review paper in Nature Reviews Molecular Cell Biology ( http://www.nature.com/nrm/index.html

), being sure to include complete article titles for all references.

Thus, there are 400 points possible for PBIO 3300 students and 450 points possible for PBIO 5300 students. Typically, 93.3% and above will earn an A, 90-93.3% an A-, 86.7-90% a B+, 83.3-86.7% a B,

80-83.3% a B-, 76.7-80% a C+, 73.3-76.7% a C, 70-73.3% a C-, 66.7-70% a D+, 63.3-66.7% a D, 60-

63.3% a D-, and below 60% an F.

Exams will be based upon material covered in class lectures as well as in the assigned readings and homework problems.

Office Hours:

By appointment, Porter Hall-Room 504 (phone: 740-593-1135 or email: showalte@ohio.edu

)

Academic Conduct/Misconduct:

The penalty for course-related academic dishonesty (i.e., cheating on exams, plagiarism, etc.) will be failure of the entire course along with a report of the incident being sent to University Judiciaries. Note that University Judiciaries may impose additional sanctions, and students may appeal academic sanctions through the grade appeal process. Cell phones must be turned off during class; violators will be asked to bring treats for the class.

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Attendance Policy:

Attendance is highly recommended. Any absences must be well justified and explained to the instructor in advance in order to make up any of the graded material.

Intellectual Property:

The lectures, classroom activities, and all materials associated with this class and developed by the instructor are copyrighted in the name of Allan Showalter.

Accommodations for Students with a Disability Policy:

Any student who suspects s/he may need an accommodation based on the impact of a disability should contact the class instructor privately to discuss the student’s specific needs and provide written documentation from the Office of Student Accessibility Services. If the student is not yet registered as a student with a disability, s/he should contact the Office of Student Accessibility Services.

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PBIO 3300/5300: PLANT GENETICS

Syllabus - Fall 2015

8

6

7

4

5

WEEK

1

2

3

14

15

12

13

9

10

Review

11

DATE

Aug. 24

Aug. 26

Aug. 28

Aug. 31

Sept. 2

Sept. 4

Sept. 7

Sept. 9

Sept. 11

Sept. 14

Sept. 16

Sept. 18

Sept. 21

Sept. 23

Sept. 25

Sept. 28

Sept. 30

Oct. 2

Oct. 5

Oct. 7

Oct. 9

Oct. 12

Oct. 14

Oct. 16

Oct. 19

Oct. 21

Oct. 23

Oct. 26

Oct. 28

Oct. 30

Nov. 2

Nov. 4

Nov. 6

Nov. 9

Nov. 11

Nov. 13

Nov. 16

Nov. 18

Nov. 20

Nov. 23

Nov. 25

Nov. 27

Nov. 30

Dec. 2

Dec. 4

Dec. 9 (Wed.)

-

15

16

16

17

18

No Class

18

19

19

20

20

No Class

No Class

-

-

-

9

10

10

12

12

6

6

-

-

7

7

2

3

3

CHAPTER TOPICS

1 Introduction; The genetics revolution

1; pp. 793-809 Genetic model organisms; genetic nomenclature

2 Single–gene inheritance

Single–gene inheritance

Independent assortment of genes

Independent assortment of genes

No Class

4

4

Mapping eukaryotic chromosomes by recombination

Mapping eukaryotic chromosomes by recombination

Gene interaction

Gene interaction

Review

8

8

No Class

9

Exam I

DNA structure and replication

DNA structure and replication

RNA: transcription and processing

RNA: transcription and processing

Proteins and their synthesis

Proteins and their synthesis

Gene isolation and manipulation

Gene isolation and manipulation

Regulation of gene expression in eukaryotes

Regulation of gene expression in eukaryotes

13

14

14 pp. 810-811

The genetic control of development

Genomes and genomics

Genomes and genomics

Bioinformatic resources for genetics & genomics;

Exam II

The dynamic genome: transposable elements

Mutation, repair, and recombination

Mutation, repair, and recombination

Large scale genomic changes

Population genetics

Population genetics

The inheritance of complex traits

The inheritance of complex traits

Evolution of genes and traits

Evolution of genes and traits

Plant breeding systems

Macroevolutionary processes

Review

Final Exam (8:00 A.M.)

3

7

8

4

6

9

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PBIO 3300/5300: PLANT GENETICS

Homework Problems from Introduction to Genetic Analysis (11th edition)

Complete and submit the assigned homework problems listed for each chapter on Mondays in class (or at the very next class meeting in the case of any holidays). Submit your answers as a printed word document; additional graphical information can be added by hand as needed. Once chapters are covered or introduced in lecture, homework assignments for those chapters will be due on the next Monday. Due dates may be adjusted by the instructor if necessary. Each chapter homework assignment is worth 5 points and two additional homework assignments (see bottom of this page) are worth 5 points each, resulting in 100 possible points for homework.

Chapter

1

Homework Problems (Assigned problems will be uploaded this Friday)

1, 4, 6, 8, 9, 11, 12, 13, 15, 19

2

3

2, 6, 10, 16, 27, 33, 35, 40, 43, 59

12, 13, 18, 20, 24, 27, 29, 33, 36, 51

12, 13, 14, 15, 17, 18, 24, 26, 31, 33

2, 5, 8, 16, 17, 18, 25, 32, 39, 46

3, 4, 8, 16, 21, 25, 28, 30, 33, 34

1, 6, 11, 13, 17, 18, 22, 24, 26, 30

12, 13, 22, 28, 33, 34, 36, 38, 43, 47

7, 10, 12, 14, 15, 19, 21, 26, 28, 32

12

13

14

15

16

17

2, 12, 15, 19, 20, 22, 24, 33, 34, 40

1, 2, 3, 6, 14

3, 4, 5, 10, 20, 22, 27, 28, 30, 32

1, 6, 9, 10, 12, 13, 14, 21, 24, 35

2, 3, 9, 10, 14, 15, 16, 19, 21, 23

1, 2, 3, 11, 22, 23, 32, 33, 41, 43

18

19

5, 7, 8, 9, 10, 11, 13, 18, 20, 23

7, 9, 10, 11, 14

20 2, 7, 8, 9, 10, 23

Two additional homework assignments are to be completed and submitted any time during the semester. These assignments should be printed as word documents and submitted in class.

1. Who is your favorite geneticist? Justify your answer in 1 page. (5 points).

2. Write a 1 page summary of a genetics-based seminar that you attend this semester. (5 points).

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