PROFESSOR: DEAN JOHNSTON OFFICE: E027 OFFICE HOURS: T 1:00 - 3:00W 12:00-1:30, Th 11:00 - 1:00 email: [email protected] PHONE: 481-4356 or 263-5618 DATE LABORATORY Aug 28 Introduction to the lab and Contamination of Cultures Sept 4 Preparation of tissue culture media Sept 11 Quantitative cellular measures Sept 25 Passage, freezing, and maintaining cells Oct 2 Thawing cells and serum requirements Oct 16 Cell fractionation and enzymatic activity Oct 23 Growth factors Oct 30 Cell cycle studies Nov 6 in vivo passage of tumor cells part I: inoculation of mice Nov 13 Primary murine fibroblasts Nov 20 Monoclonal antibodies Nov 27 in vivo passage of tumor cells Dec 4 Dec 11 part II: isolation of cells Chromosomal analysis ELISA ATTENDANCE POLICY: Students are responsible for all work in this course No unexcused absence or tardiness will be permitted Attendance will be taken at the beginning of the class In this course the labs cannot be made up. For any excused absence you must contact me before the next class to arrange for an alternate assignment. Course Web Page Cell & Tissue Culture will have an informational file on the Hunter College computer system. To access this file go to the Hunter home page (hunter.cuny.edu). At the home page click on Student Services then on Course Web Pages. From here search by course number for 450. Click on MLS 450. This course is code protected. The code is mls450.