BIOGRAPHICAL SKETCH Provide the following information for the Senior/key personnel and other significant contributors in the order listed on Form Page 2. Follow this format for each person. DO NOT EXCEED FOUR PAGES. NAME POSITION TITLE Gregg E. Homanics, PhD Professor of Anesthesiology and Pharmacology & Chemical Biology eRA COMMONS USER NAME (credential, e.g., agency login) homanicsge EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, include postdoctoral training and residency training if applicable.) DEGREE INSTITUTION AND LOCATION MM/YY FIELD OF STUDY (if applicable) Penn. State Univ.(Fayette Campus) West Virginia Univ. (Morgantown, WV) Univ. Kentucky (Lexington, KY) North Carolina State Univ. (Raleigh, NC) Univ. of North Carolina (Chapel Hill, NC) -- 12/80 BS MS PhD postdoc. 05/84 07/87 05/91 05/93 Pre-Medicine Animal and Vet. Sci. Animal Science Animal Science Molecular Genetics A. Personal Statement As a postdoctoral fellow at UNC-CH in the labs of N. Maeda and O. Smithies (Nobel Prize, 2007), I conducted pioneering studies in the development of gene knockout mice. Since moving to the U. of Pittsburgh, I have focused my efforts on using genetically modified animals to investigate the molecular mechanisms of action of ethanol and general anesthetics. In addition to creating numerous global gene knockout, transgenic, knockin, and conditional knockout mouse lines, I also pioneered an approach for creating conditional gene knockin mice. I also have an extensive track record of successfully using my mouse genetic engineering skills in collaborations with other investigators in a variety of research areas that are beyond the primary focus of my laboratory. B. Positions and Honors Positions and Employment Dates 1/86-7/87 8/87-3/91 4/91-5/93 6/93-7/01 7/01-7/08 8/08-present Position Graduate assistant Graduate assistant Postdoctoral fellow Assist. Professor Assoc. Professor Professor Institution Univ. Kentucky North Carolina State U. Univ. North Carolina Univ. Pittsburgh Univ. Pittsburgh Univ. Pittsburgh Department Animal Science Animal Science Pathology Anesthesiology/CCM and Pharm. Anesthesiology and Pharmacology Anesthesiology and Pharm. & Chem. Biol. Honors and Other Professional Activities 1991-1993 National Research Service Award Individual Postdoctoral Fellowship, NIH 1999 University of Pittsburgh Distinguished Mentor Award 1999-present University of Pittsburgh IACUC (2001-2004, Chair) 2002-2005 National Institutes of Health, AA-1 regular member 2005-present Faculty of 1000 Medicine, Evaluation Board Member, Anesthesiology and Pain Management 2005 National Institute of Mental Health, Board of Scientific Counselors, ad hoc reviewer 2006-present Alcohol, editorial board member 2008-present Frontiers in Molecular Neuroscience, review editor 2010-2014 National Institutes of Health, Neurotoxicology and Alcohol Study Section -Regular member (chair 2012-2014) 2010 National Institutes of Health MERIT Award C. Selected peer-reviewed publications (selected from 123 peer-reviewed publications) Homanics GE, Smith TJ, Zhang SH, Lee D, Young SG, Maeda N (1993) Targeted modification of the apolipoprotein B gene results in hypobetalipoproteinemia and developmental abnormalities in mice. Proc Natl Acad Sci USA 90:2389-2393. Huntsman MM, Porcello DM, Homanics GE, DeLorey TM, Huguenard JR (1999) Reciprocal inhibitory connections control network synchrony in the mammalian thalamus. Science 283:541-543. Chandra D, Jia F, Liang J, Peng Z, Suryanarayanan A, Werner DF, Spigelman I, Houser CR, Olsen RW, Harrison NL, Homanics GE (2006) GABAA receptor α4 subunits mediate extrasynaptic inhibition in thalamus and dentate gyrus and the action of gaboxadol. Proc Natl Acad Sci USA 103:15230-15235. Skvorak K, Vissel B, Homanics GE (2006) Production of conditional point mutant knockin mice. Genesis 44:345-353. Werner DF, Blednov YA, Borghese CM, Henderson LA, Ariwodola OJ, Silberman Y, Logan E, Berry RB, Matthews DB, Weiner JL, Harrison NL, Harris RA, Homanics GE (2006) Knockin mice harboring ethanol insensitive alpha1containing GABAA receptors display selective alterations in behavioral responses to ethanol. J Pharmacol Exp Ther 319:219-227. Ferguson C, Hardy SL, Werner DF, Hileman SM, DeLorey TM, Homanics GE (2007) New insight into the role of the β3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout. BMC Neurosci:85. Glykys J, Peng Z, Chandra D, Homanics GE, Houser CR, Mody I (2007) A novel naturally occuring GABAA receptor partnership with high sensitivity to ethanol. Nat Neurosci 10:40-48. Chandra D, Werner DF, Olsen RW, Harrison NL, Homanics GE (2008) Normal acute behavioral responses to moderate/high dose ethanol in GABAA receptor α4 subunit knockout mice. Alcohol Clin Exp Res 32:10-18. PMCID: PMC2896280. Blednov YA, Walker DL, Iyer SV, Homanics G, Harris AR (2010) Mice lacking Gad2 show altered behavioral effects of ethanol, flurazepam and gabaxadol. Addict Biol 15:45-61. PMCID: PMC PMC3038569. Haselkorn ML, Shellington D, Jackson E, Vagni VA, Janesko KL, Dubey RK, Gillespie DG, Cheng D, Bell MJ, Jenkins LW, Homanics GE, Schnermann J, Kochanek PM (2010) Adenosine A1 receptor activation as a brake on the microglial response after experimental traumatic brain injury in mice. J Neurotrauma 27:901-910. PMCID: PMC2943944. Jentarra GM, Olfers SL, Rice SG, Srivastava N, Homanics GE, Blue M, Naidu S, Narayanan V (2010) Abnormalities of cell packing density and dendritic complexity in the MeCP2 A140V mouse model of Rett syndrome/X-linked mental retardation. BMC Neurosci 11:19. PMCID: PMC 2836362. Wilking JA, Hesterberg KG, Crouch EL, Homanics GE, Stitzel JA (2010) Chrna4 A529 knock-in mice exhibit altered nicotine sensitivity. Pharmacogenet Genomics 20:121-130. PMCID: PMC2919848. Blednov YA, Borghese CM, McCracken ML, Benavidez JM, Geil CR, Osterndorff-Kahanek E, Werner DF, Iyer S, Swihart A, Harrison NL, Homanics GE, Harris RA (2011) Loss of ethanol conditioned taste aversion and motor stimulation in knockin mice with ethanol-insensitive alpha2-containing GABA(A) receptors. J Pharmacol Exp Ther 336:145154. PMCID: PMC 3014308. Werner DF, Swihart A, Rau V, Jia F, Borghese CM, McCracken ML, Iyer S, Fanselow MS, Oh I, Sonner JM, Eger EI, 2nd, Harrison NL, Harris RA, Homanics GE (2011) Inhaled anesthetic responses of recombinant receptors and knockin mice harboring alpha2(S270H/L277A) GABA(A) receptor subunits that are resistant to isoflurane. J Pharmacol Exp Ther 336:134-144. PMCID: PMC 3014300. Blednov YA, Benavidez JM, Homanics GE, Harris RA (2012) Behavioral characterization of KI mice with mutations M287L and Q266I in the GlyR 1 subunit. J Pharmacol Exp Ther 340: 317-329. PMCID: PMC3263963. Borghese CM, Blednov YA, Quan Y, Iyer SV, Xiong W, Mihic SJ, Zhang L, Lovinger DM, Trudell JR, Homanics GE, Harris RA (2012) Characterization of two mutations, M287L and Q266I, in the 1 glycine receptor subunit that modify sensitivity to alcohols and anesthetics. J Pharmacol Exp Ther 340: 304-316. PMCID: PMC3263968. Borghese CM, Xiong W, Oh SI, Ho A, Mihic SJ, Zhang L, Lovinger DM, Homanics GE, Eger EI, 2nd, Harris RA (2012) Mutations M287L and Q266I in the Glycine Receptor alpha1 Subunit Change Sensitivity to Volatile Anesthetics in Oocytes and Neurons, but Not the Minimal Alveolar Concentration in Knockin Mice. Anesthesiology 117:765-771. PMCID: PMC 3447119. Sabaliauskas N, Shen H, Homanics GE, Smith SS, Aoki C (2012) Knockout of the gamma-aminobutyric acid receptor subunit alpha4 reduces functional delta-containing extrasynaptic receptors in hippocampal pyramidal cells at the onset of puberty. Brain Res 1450:11-23. PMCID: PMC 3319511.