University of Pennsylvania
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
Web: www.med.upenn.edu/dnaseq/
DNA SEQUENCING FACILITY
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
FTP: dnaseq.med.upenn.edu
Request For Ion Torrent PGM Sequencing Service
Sequencing kits of both 200 b & 400 b read lengths are available.
PI ____________________________
Fund/PO No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Ion Torrent PGM sequencer combines pre-existing semiconductor technology with a simple sequencing
chemistry ( http://www.iontorrent.com ). The sequencer is essentially a solid state pH meter. Known for scalability,
simplicity and speed, this inexpensive technology is really moving fast to achieve new goals in terms of throughput
and read length.
Applications include: Targeted resequencing of barcoded samples; Sequencing of captured
library(captured region 100 Kb to 1 Mb) using 316 chip; Sequencing of bacterial and viral genomes; Sequencing
of metagenomic samples; RNA-seq specially small RNA sequencing; Validation of sequence data obtained on
other platforms.
Only 1 sample is run at a time. No waiting is necessary for a plate/flow cell/slide to fill up.
Expected Run Results (400 b sequencing only on 314 chip currently)
314 chip
316 chip
318 chip
Read Length
200b
400b
200b
200b
No of Reads*
> 100,000
> 100,000
> 1M
~5 M
Throughput*
> 20 Mb
> 40 Mb
> 200 Mb
~ 1 Gb
* The throughputs on 314 and 316 chips are 3-4 fold higher than specifications. For 318 chip it’s slightly
higher.
Price Effective Apr 1, 2013
Sequencing Chip
314
316
318
* gDNA (& also long amplicons,
includes 1 library prep)
$600 (200b)
$725 (400b)
$800 (200b)
$995 (200b)
§
$515 (200b)
$640 (400b)
$720 (200b)
$920 (200b)
Amplicons (library with adaptors already made)
* AmpliSeq Cancer panel v2
(50 cancer genes, 2,800 COSMIC mutations,
includes 1 library prep)
AmpliSeq Comprehensive Cancer & other panels
$700 (200b) $900 (200b)
Contact us for multiplexing
Contact us
Barcoded library - $80 (gDNA, amplicons
$170 (for AmpliSeq cancer or custom panel,
4 minimum)
4 minimum)
*The price includes 1 library prep (for gDNA, long amplicons and AmpliSeq panels), sample evaluation,
emPCR, sequencing on a chip, and preliminary data analysis to provide BAM and BAI files.
§
The price does not include the preparation of amplicon library with Ion adaptors but includes sample evaluation.
04/01/13
Ion Torrent PGM service
Please Fill Out Template
Template
Name
Type
Size
Amount**
Concentration
(µg)
(ng/µl)
Sequencing primer
1.
2.
3.
4.
5.
** gDNA or pooled amplicons
Required 300 - 400 ng by fluorometry or 1 – 1.5 µg by Nanodrop, Volume 30 – 50 µl
** Amplicon library with Ion adaptors attached
Required 100 – 200 ng by fluorometry or 300 - 600 ng by Nanodrop, Volume 30 –50 µl
Please Check the FollowingGel Picture Submitted (
)
Quantitation Method: Nanodrop (
Samples Pooled:
)
Barcoded:
(
Throughput Desired for Each Sample
(
) or Fluorometry (
)
)
No of reads _______________, No of bases _____________
Use 314 chip (
)
316 chip (
)
Use 200 b kit (
)
400 b kit (
), 314 chip only
318 chip (
)
Note: Please mention barcode sequences in the project description if different from Ion Torrent’s.
Project description with a title (required)
use separate page if necessary
--------------------
04/01/13